Determination of sialic acids released from glycoproteins using capillary zone electrophoresis/electrospray ionization mass spectrometry

被引：21
|
作者：
Ortner, Karin [1 ]
Buchberger, Wolfgang [1 ]
机构：
[1] Johannes Kepler Univ Linz, Inst Analyt Chem, A-4040 Linz, Austria
来源：
ELECTROPHORESIS | 2008年 / 29卷 / 10期
关键词：
capillary zone electrophoresis-mass spectrometry; glycoproteins; N-acetylneuraminic acid; N-glycolyineuraminic acid; sialic acids;
D O I：
10.1002/elps.200700801
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple method for the determination of the two most abundant sialic acids released from glycoproteins based on CZE-MS is presented. Several parameters like BGE with various organic modifiers and sheath liquids were studied with respect to their suitability for the fast and easy analysis of the selected compounds by CZE-MS. Finally, a BGE containing 10 mM ammonium acetate allowed the quantification of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) in glycoproteins as well as human plasma samples. LOD and LOQ were 2 mu g/mL and 6 mu g/mL, respectively.
引用
收藏
页码：2233 / 2237
页数：5
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