Nontuberculous mycobacteria - clinical and laboratory diagnosis: experiences from a TB endemic country

被引：5

作者：

Sam, Ann Susan ^{[1
]}

Ninan, Marilyn Mary ^{[1
]}

Ranjani, R. ^{[1
]}

Raghupathi, Naveen Kumar Devanga ^{[1
]}

Balaji, Veeraraghavan ^{[1
]}

Michael, Joy Sarojini ^{[1
]}

机构：

[1] Christian Med Coll & Hosp, Dept Clin Microbiol, Vellore 632004, Tamil Nadu, India

来源：

FUTURE SCIENCE OA | 2020年 / 6卷 / 09期

关键词：

geographic distribution; identification; MALDI-TOF mass spectrometry; nontuberculous mycobacteria; prevalence; sequencing; VITEK(R)MS; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; IDENTIFICATION; DISEASE; EPIDEMIOLOGY; INFECTION; COMPLEX; OREGON; USA;

D O I：

10.2144/fsoa-2020-0023

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aim:To evaluate the performance of VITEK(R)MS with DNA sequencing for laboratory diagnosis of non-tuberculous mycobacteria (NTM) species in a resource-limited setting.Methods:16SrRNAsequencing and MALDI-TOF mass spectrometry (VITEK(R)MS) was performed at a tertiary-care hospital in India. MALDI-TOF results were confirmed by 16S rRNA sequencing. In addition, sequencing of the internal transcribed spacer region was performed on slowly growing NTM.Results:Commonest species isolated wereM. abscessus, M. intracellulare,M. avium, M. fortuitumandM. simiae.16S rRNAsequencing and MALDI-TOF results had agreement of 94.5% for rapidly growing and 77.5% for slowly growing NTM.Conclusion:There is good correlation between VITEK(R)MS and sequencing for rapidly growing NTM. For slowly growing species, sequencing would be required in a third isolates.

引用

页数：10

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