Quinoline-Based Two-Photon Fluorescent Probe for Nitric Oxide in Live Cells and Tissues

被引：96
作者：
Dong, Xiaohu [1 ,3 ]
Heo, Cheol Ho [2 ]
Chen, Shiyu [1 ]
Kim, Hwan Myung [2 ]
Liu, Zhihong [1 ]
机构：
[1] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China
[2] Ajou Univ, Div Energy Syst Res, Suwon 443749, Gyeonggi Do, South Korea
[3] Xian Modern Chem Res Inst, Xian 710065, Shaanxi, Peoples R China
来源：
ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 01期
基金：
中国国家自然科学基金;
关键词：
AUTOPHAGY; PEROXYNITRITE; INDICATORS; HEALTH; NO;
D O I：
10.1021/ac403226h
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A two-photon fluorescent probe (QNO) for nitric oxide is reported. The probe is designed with a photoinduced electron transfer (PeT) mechanism and shows 12-fold fluorescence enhancement toward NO. Adopting a quinoline derivative as the fluorophore, QNO has a large two-photon action cross section value of 52 GM and long-wavelength emission. It also features high selectivity, low cytotoxicity, and pH insensitivity. By utilizing two-photon microscopy (TPM), QNO can detect NO in live cells and live tissues at a depth of 90-180 mu m.
引用
收藏
页码：308 / 311
页数：4
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