Attomolar DNA detection with chiral nanorod assemblies

被引:492
作者
Ma, Wei [1 ]
Kuang, Hua [1 ]
Xu, Liguang [1 ]
Ding, Li [2 ]
Xu, Chuanlai [1 ]
Wang, Libing [1 ,2 ]
Kotov, Nicholas A. [3 ,4 ,5 ,6 ]
机构
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] State Key Lab Food Safety Test Hunan, Changsha 410004, Hunan, Peoples R China
[3] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Dept Mat Sci, Ann Arbor, MI 48109 USA
[5] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[6] Univ Michigan, Biointerface Inst, Ann Arbor, MI 48109 USA
基金
中国国家自然科学基金;
关键词
ENHANCED RAMAN-SCATTERING; POLYMERASE-CHAIN-REACTION; SIDE-BY-SIDE; GOLD NANORODS; CIRCULAR-DICHROISM; COLORIMETRIC DETECTION; ULTRASENSITIVE DNA; OPTICAL-ACTIVITY; NANOPARTICLES; SURFACE;
D O I
10.1038/ncomms3689
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Nanoscale plasmonic assemblies display exceptionally strong chiral optical activity. So far, their structural design was primarily driven by challenges related to metamaterials whose practical applications are remote. Here we demonstrate that gold nanorods assembled by the polymerase chain reaction into DNA-bridged chiral systems have promising analytical applications. The chiroplasmonic activity of side-by-side assembled patterns is attributed to a 7-9 degree twist between the nanorod axes. This results in a strong polarization rotation that matches theoretical expectations. The amplitude of the bisignate 'wave' in the circular dichroism spectra of side-by-side assemblies demonstrates excellent linearity with the amount of target DNA. The limit of detection for DNA using side-by-side assemblies is as low as 3.7 aM. The chiroplasmonic method may be particularly useful for biological analytes larger than 2-5 nm which are difficult to detect by methods based on plasmon coupling and 'hot spots'. Circular polarization increases for inter-nanorod gaps between 2 and 20 nm when plasmonic coupling rapidly decreases. Reaching the attomolar limit of detection for simple and reliable bioanalysis of oligonucleotides may have a crucial role in DNA biomarker detection for early diagnostics of different diseases, forensics and environmental monitoring.
引用
收藏
页数:8
相关论文
共 39 条
[1]   Gold nanorods 3D-supercrystals as surface enhanced Raman scattering spectroscopy substrates for the rapid detection of scrambled prions [J].
Alvarez-Puebla, Ramon A. ;
Agarwal, Ashish ;
Manna, Pramit ;
Khanal, Bishnu P. ;
Aldeanueva-Potel, Paula ;
Carbo-Argibay, Enrique ;
Pazos-Perez, Nicolas ;
Vigderman, Leonid ;
Zubarev, Eugene R. ;
Kotov, Nicholas A. ;
Liz-Marzan, Luis M. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (20) :8157-8161
[2]   Fingers Crossed: Optical Activity of a Chiral Dimer of Plasmonic Nanorods [J].
Auguie, Baptiste ;
Lorenzo Alonso-Gomez, Jose ;
Guerrero-Martinez, Andres ;
Liz-Marzan, Luis M. .
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2011, 2 (08) :846-851
[3]   Application of electronic circular dichroism in configurational and conformational analysis of organic compounds [J].
Berova, Nina ;
Di Bari, Lorenzo ;
Pescitelli, Gennaro .
CHEMICAL SOCIETY REVIEWS, 2007, 36 (06) :914-931
[4]   Supramolecular assembly facilitating adsorbate-induced chiral electronic states in a metal surface [J].
Bovet, Nicolas ;
McMillan, Nicola ;
Gadegaard, Nikolaj ;
Kadodwala, Malcolm .
JOURNAL OF PHYSICAL CHEMISTRY B, 2007, 111 (33) :10005-10011
[5]   Nanoparticle Superstructures Made by Polymerase Chain Reaction: Collective Interactions of Nanoparticles and a New Principle for Chiral Materials [J].
Chen, Wei ;
Bian, Ai ;
Agarwal, Ashish ;
Liu, Liqiang ;
Shen, Hebai ;
Wang, Libing ;
Xu, Chuanlai ;
Kotov, Nicholas A. .
NANO LETTERS, 2009, 9 (05) :2153-2159
[6]   Self-Assembly of Quantum Dots and Carbon Nanotubes for Ultrasensitive DNA and Antigen Detection [J].
Cui, Daxiang ;
Pan, Bifeng ;
Zhang, Hong ;
Gao, Feng ;
Wu, Rina ;
Wang, Jingping ;
He, Rong ;
Asahi, Toru .
ANALYTICAL CHEMISTRY, 2008, 80 (21) :7996-8001
[7]   Gold Nanoparticles with Asymmetric Polymerase Chain Reaction for Colorimetric Detection of DNA Sequence [J].
Deng, Hua ;
Xu, Yi ;
Liu, Yanhua ;
Che, Zhijun ;
Guo, Huilin ;
Shan, Shuxian ;
Sun, Yun ;
Liu, Xiaofang ;
Huang, Keyang ;
Ma, Xiaowei ;
Wu, Yan ;
Liang, Xing-Jie .
ANALYTICAL CHEMISTRY, 2012, 84 (03) :1253-1258
[8]   First enantioseparation and circular dichroism spectra of Au38 clusters protected by achiral ligands [J].
Dolamic, Igor ;
Knoppe, Stefan ;
Dass, Amala ;
Buergi, Thomas .
NATURE COMMUNICATIONS, 2012, 3
[9]   Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].
Elghanian, R ;
Storhoff, JJ ;
Mucic, RC ;
Letsinger, RL ;
Mirkin, CA .
SCIENCE, 1997, 277 (5329) :1078-1081
[10]  
Ellison S. L. R., 2012, AW EURACHEM CITAC GU