Extracellular Electron Transfer across Bacterial Cell Membranes via a Cytocompatible Redox-Active Polymer

被引：45

作者：

Nishio, Koichi ^{[2
]}

Nakamura, Ryuhei ^{[2
]}

Lin, Xiaojie ^{[3
]}

Konno, Tomohiro ^{[4
]}

Ishihara, Kazuhiko ^{[3
,4
]}

Nakanishi, Shuji ^{[1
]}

Hashimoto, Kazuhito ^{[2
]}

机构：

[1] Univ Tokyo, Res Ctr Adv Sci & Technol, Meguro Ku, Tokyo 1538904, Japan

[2] Univ Tokyo, Dept Appl Chem, Sch Engn, Bunkyo Ku, Tokyo 1138656, Japan

[3] Univ Tokyo, Dept Mat Engn, Sch Engn, Bunkyo Ku, Tokyo 1138656, Japan

[4] Univ Tokyo, Sch Engn, Dept Bioengn, Bunkyo Ku, Tokyo 1138656, Japan

来源：

CHEMPHYSCHEM | 2013年 / 14卷 / 10期

关键词：

cytotoxicity; electrochemistry; electron transfer; microbiology; phospholipids; PHOSPHOLIPID POLYMERS; LIVING CELLS; SHEWANELLA;

D O I：

10.1002/cphc.201300117

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A redox-active phospholipid polymer with a phospholipid-mimicking structure (2-methacryloyloxyethyl phosphorylcholine; MPC) was synthesized to construct a biocompatible electron mediator between bacteria and an electrode. In this study, a copolymer of MPC and vinylferrocene [VF; poly(MPC-co-VF)] (PMF) is synthesized. When PMF is added to cultures of the bacterial species Escherichia coli (Gram negative) and Lactobacillus plantarum (Gram positive), which have different cell wall structures, a catalytic current mediated by PMF is observed. In addition, growth curves and live/dead assays indicate that PMF does not decrease metabolic activity or cell viability. These results indicate that PMF mediates extracellular electron transfer across bacterial cell membranes without associated cytotoxicity.

引用

页码：2159 / 2163

页数：5

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