Development and validation of a High Performance Liquid Chromatography-Diode Array Detection method for the determination of aging markers in tequila

被引：23

作者：

Celia Munoz-Munoz, Ana ^{[1
]}

Charles Grenier, Adam ^{[2
]}

Gutierrez-Pulido, Humberto ^{[3
]}

Cervantes-Martinez, Jesus ^{[1
]}

机构：

[1] Ctr Invest & Asistencia Tecnol & Diseno Estado Ja, Guadalajara 44270, Jalisco, Mexico

[2] Peace Corps Mexico, Queretaro 76020, Mexico

[3] Univ Guadalajara, Dept Matemat, Ctr Univ Ciencias Exactas & Ingn, Guadalajara 44840, Jalisco, Mexico

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1213卷 / 02期

关键词：

Tequila; Aging; HPLC-DAD; Oak related compounds; Oak barrels; Discriminant analysis;

D O I：

10.1016/j.chroma.2008.10.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A solid-phase extraction procedure followed by analysis by high performance liquid chromatography (HPLC) with UV-vis photodiode array detection (DAD) is proposed to simultaneously determine 11 aging markers in tequila. The method showed good intraday (n = 5) and interday (n = 3) precision, RSD < 1.6% in both cases, for each of the identified compounds. The calibration curves were linear at the tested ranges (R-2 > 0.999). Good recoveries (84.2-108.5%) were obtained for 10 of the I I compounds studied; and the LOD and LOQ ranged from 0.62 to 4.09 mu g/mL and 1.9-12.4 mu g/mL, respectively. The proposed methodology was applied to a set of 15 authentic tequila samples grouped by aging state (blanco, reposado and anejo). An ANOVA analysis combined with discriminant analysis with stepwise backward variable selection was used to differentiate between the various aging groups based on their oak related compounds content. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：218 / 223

页数：6

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