Sperm, but Not Oocyte, DNA Methylome Is Inherited by Zebrafish Early Embryos

被引：371

作者：

Jiang, Lan ^{[1
,3
]}

Zhang, Jing ^{[1
]}

Wang, Jing-Jing ^{[1
,3
]}

Wang, Lu ^{[1
,3
]}

Zhang, Li ^{[1
]}

Li, Guoqiang ^{[1
,3
]}

Yang, Xiaodan ^{[2
]}

Ma, Xin ^{[1
,3
]}

Sun, Xin ^{[1
]}

Cai, Jun ^{[1
]}

Zhang, Jun ^{[4
]}

Huang, Xingxu ^{[4
]}

Yu, Miao ^{[5
,6
]}

Wang, Xuegeng ^{[7
,8
]}

Liu, Feng ^{[2
]}

Wu, Chung-I ^{[1
]}

He, Chuan ^{[5
,6
]}

Zhang, Bo ^{[7
,8
]}

Ci, Weimin ^{[1
]}

Liu, Jiang ^{[1
]}

机构：

[1] Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Inst Zool, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100101, Peoples R China

[3] Beijing Univ, Chinese Acad Sci, Beijing 100049, Peoples R China

[4] Nanjing Univ, Model Anim Res Ctr, Nanjing 210061, Peoples R China

[5] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

[6] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA

[7] Peking Univ, Key Lab Cell Proliferat, Beijing 100871, Peoples R China

[8] Peking Univ, Coll Life Sci, Differentiat Minist Educ, Beijing 100871, Peoples R China

来源：

CELL | 2013年 / 153卷 / 04期

基金：

美国国家卫生研究院; 中国国家自然科学基金;

关键词：

TRANSGENERATIONAL EPIGENETIC INHERITANCE; GENOMIC METHYLATION; 5-HYDROXYMETHYLCYTOSINE; DEMETHYLATION; EXPRESSION;

D O I：

10.1016/j.cell.2013.04.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5-methylcytosine is a major epigenetic modification that is sometimes called "the fifth nucleotide.'' However, our knowledge of how offspring inherit the DNA methylonne from parents is limited. We generated nine single-base resolution DNA methylomes, including zebrafish gametes and early embryos. The oocyte methylome is significantly hypomethylated compared to sperm. Strikingly, the paternal DNA methylation pattern is maintained throughout early embryogenesis. The maternal DNA methylation pattern is maintained until the 16-cell stage. Then, the oocyte methylome is gradually discarded through cell division and is progressively reprogrammed to a pattern similar to that of the sperm methylonne. The passive demethylation rate and the de novo methylation rate are similar in the maternal DNA. By the midblastula stage, the embryo's methylome is virtually identical to the sperm methylome. Moreover, inheritance of the sperm methylonne facilitates the epigenetic regulation of embryogenesis. Therefore, besides DNA sequences, sperm DNA methylome is also inherited in zebrafish early embryos.

引用

页码：773 / 784

页数：12

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