RAPD Analysis to Study Metagenome Diversity in Soil Microbial Community of Arid Zone Plants

被引：4
作者：
Sharma, G. [1 ]
Verma, H. N. [1 ]
Sharma, R. [2 ]
机构：
[1] Jaipur Natl Univ, Dept Life Sci, Jaipur, Rajasthan, India
[2] Rajasthan Agr Univ, Dept Plant Biotechnol, Bikaner, Rajasthan, India
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES INDIA SECTION B-BIOLOGICAL SCIENCES | 2013年 / 83卷 / 02期
关键词：
RAPD analysis; Metagenome diversity; Arid zone; Soil microbial community; Rhizosphere; PCR; BACTERIAL DIVERSITY; GROWTH;
D O I：
10.1007/s40011-012-0041-1
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The study was conducted by taking six soil samples, two each for Capparis decidua (Ker), Calligonum polygonoides (Phog) collected from Bikaner while third sample, Cuminum cyminum (Cumin) was collected from Jobner. RAPD analysis was done using various decamer primers. The total bands amplified varied from 7 (UBC 153) to 13 (UBC 157). Three primers (UBC 153, 154 and 162) showed 100 % polymorphism. 46 out of 53 polymorphic allelic positions were obtained with 86.7 % polymorphism. The number of bands generated was plant and soil dependent.
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收藏
页码：135 / 139
页数：5
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