Genotypes and Mouse Virulence of Toxoplasma gondii Isolates from Animals and Humans in China

被引：103

作者：

Wang, Lin ^{[1
,2
,4
]}

Chen, He ^{[3
]}

Liu, Daohua ^{[5
]}

Huo, Xingxing ^{[3
]}

Gao, Jiangmei ^{[6
]}

Song, Xiaorong ^{[1
,2
]}

Xu, Xiucai ^{[1
,2
]}

Huang, Kaiquan ^{[4
]}

Liu, Wenqi ^{[7
]}

Wang, Yong ^{[8
]}

Lu, Fangli ^{[9
,10
]}

Lun, Zhao-Rong ^{[6
]}

Luo, Qingli ^{[1
,2
]}

Wang, Xuelong ^{[1
,2
]}

Shen, Jilong ^{[1
,2
]}

机构：

[1] Anhui Med Univ, Dept Parasitol, Prov Lab Microbiol & Parasitol, Hefei, Anhui, Peoples R China

[2] Anhui Med Univ, Key Lab Zoonoses Anhui, Hefei, Anhui, Peoples R China

[3] Anhui Med Univ, Affiliated Hosp 1, Clin Lab, Hefei, Anhui, Peoples R China

[4] Anhui Univ Tradit Chinese Med, Affiliated Hosp 1, Clin Lab, Hefei, Peoples R China

[5] Prov Inst Parasit Dis Control Anhui, Hefei, Anhui, Peoples R China

[6] Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Key Lab Trop Dis Control,Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

[7] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Parasitol, Wuhan 430074, Hubei, Peoples R China

[8] Nanjing Med Univ, Dept Pathogen Biol, Nanjing, Jiangsu, Peoples R China

[9] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

[10] Sun Yat Sen Univ, Zhongshan Sch Med, Key Lab Trop Dis Control, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 01期

关键词：

GENETIC-CHARACTERIZATION; POPULATION-STRUCTURE; CONGENITAL TOXOPLASMOSIS; CLONAL LINEAGES; HIGH PREVALENCE; NORTH-AMERICA; SOUTH-AMERICA; STRAINS; IDENTIFICATION; CATS;

D O I：

10.1371/journal.pone.0053483

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Recent population structure studies of T. gondii revealed that a few major clonal lineages predominated in different geographical regions. T. gondii in South America is genetically and biologically divergent, whereas this parasite is remarkably clonal in North America and Europe with a few major lineages including Types I, II and III. Information on genotypes and mouse virulence of T. gondii isolates from China is scarce and insufficient to investigate its population structure, evolution, and transmission. Methodology/Principal Findings: Genotyping of 23 T. gondii isolates from different hosts using 10 markers for PCR-restriction fragment length polymorphism analyses (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) revealed five genotypes; among them three genotypes were atypical and two were archetypal. Fifteen strains belong to the Chinese 1 lineage, which has been previously reported as a widespread lineage from swine, cats, and humans in China. Two human isolates fall into the type I and II lineages and the remaining isolates belong to two new atypical genotypes (ToxoDB#204 and #205) which has never been reported in China. Our results show that these genotypes of T. gondii isolates are intermediately or highly virulent in mice except for the strain TgCtwh6, which maintained parasitemia in mice for 35 days post infection although it possesses the uniform genotype of Chinese 1. Additionally, phylogenetic network analyses of all isolates of genotype Chinese 1 are identical, and there is no variation based on the sequence data generated for four introns (EF1, HP2, UPRT1 and UPRT7) and two dense granule proteins (GRA6 and GRA7). Conclusion/Significance: A limited genetic diversity was found and genotype Chinese 1 (ToxoDB#9) is dominantly circulating in mainland China. The results will provide a useful profile for deep insight to the population structure, epidemiology and biological characteristics of T. gondii in China.

引用

页数：11

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