Genotypes and Mouse Virulence of Toxoplasma gondii Isolates from Animals and Humans in China

被引:104
作者
Wang, Lin [1 ,2 ,4 ]
Chen, He [3 ]
Liu, Daohua [5 ]
Huo, Xingxing [3 ]
Gao, Jiangmei [6 ]
Song, Xiaorong [1 ,2 ]
Xu, Xiucai [1 ,2 ]
Huang, Kaiquan [4 ]
Liu, Wenqi [7 ]
Wang, Yong [8 ]
Lu, Fangli [9 ,10 ]
Lun, Zhao-Rong [6 ]
Luo, Qingli [1 ,2 ]
Wang, Xuelong [1 ,2 ]
Shen, Jilong [1 ,2 ]
机构
[1] Anhui Med Univ, Dept Parasitol, Prov Lab Microbiol & Parasitol, Hefei, Anhui, Peoples R China
[2] Anhui Med Univ, Key Lab Zoonoses Anhui, Hefei, Anhui, Peoples R China
[3] Anhui Med Univ, Affiliated Hosp 1, Clin Lab, Hefei, Anhui, Peoples R China
[4] Anhui Univ Tradit Chinese Med, Affiliated Hosp 1, Clin Lab, Hefei, Peoples R China
[5] Prov Inst Parasit Dis Control Anhui, Hefei, Anhui, Peoples R China
[6] Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Key Lab Trop Dis Control,Minist Educ, Guangzhou 510275, Guangdong, Peoples R China
[7] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Parasitol, Wuhan 430074, Hubei, Peoples R China
[8] Nanjing Med Univ, Dept Pathogen Biol, Nanjing, Jiangsu, Peoples R China
[9] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China
[10] Sun Yat Sen Univ, Zhongshan Sch Med, Key Lab Trop Dis Control, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China
关键词
GENETIC-CHARACTERIZATION; POPULATION-STRUCTURE; CONGENITAL TOXOPLASMOSIS; CLONAL LINEAGES; HIGH PREVALENCE; NORTH-AMERICA; SOUTH-AMERICA; STRAINS; IDENTIFICATION; CATS;
D O I
10.1371/journal.pone.0053483
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Recent population structure studies of T. gondii revealed that a few major clonal lineages predominated in different geographical regions. T. gondii in South America is genetically and biologically divergent, whereas this parasite is remarkably clonal in North America and Europe with a few major lineages including Types I, II and III. Information on genotypes and mouse virulence of T. gondii isolates from China is scarce and insufficient to investigate its population structure, evolution, and transmission. Methodology/Principal Findings: Genotyping of 23 T. gondii isolates from different hosts using 10 markers for PCR-restriction fragment length polymorphism analyses (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) revealed five genotypes; among them three genotypes were atypical and two were archetypal. Fifteen strains belong to the Chinese 1 lineage, which has been previously reported as a widespread lineage from swine, cats, and humans in China. Two human isolates fall into the type I and II lineages and the remaining isolates belong to two new atypical genotypes (ToxoDB#204 and #205) which has never been reported in China. Our results show that these genotypes of T. gondii isolates are intermediately or highly virulent in mice except for the strain TgCtwh6, which maintained parasitemia in mice for 35 days post infection although it possesses the uniform genotype of Chinese 1. Additionally, phylogenetic network analyses of all isolates of genotype Chinese 1 are identical, and there is no variation based on the sequence data generated for four introns (EF1, HP2, UPRT1 and UPRT7) and two dense granule proteins (GRA6 and GRA7). Conclusion/Significance: A limited genetic diversity was found and genotype Chinese 1 (ToxoDB#9) is dominantly circulating in mainland China. The results will provide a useful profile for deep insight to the population structure, epidemiology and biological characteristics of T. gondii in China.
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页数:11
相关论文
共 51 条
[1]   Chronic Toxoplasma Infection Modifies the Structure and the Risk of Host Behavior [J].
Afonso, Cristina ;
Paixao, Vitor B. ;
Costa, Rui M. .
PLOS ONE, 2012, 7 (03)
[2]   Type I strains in human toxoplasmosis: myth or reality? [J].
Ajzenberg, Daniel .
FUTURE MICROBIOLOGY, 2010, 5 (06) :841-843
[3]   The Distribution of Toxoplasma gondii Cysts in the Brain of a Mouse with Latent Toxoplasmosis: Implications for the Behavioral Manipulation Hypothesis [J].
Berenreiterova, Miroslava ;
Flegr, Jaroslav ;
Kubena, Ales A. ;
Nemec, Pavel .
PLOS ONE, 2011, 6 (12)
[4]   Effects of Toxoplasma gondii infection on the brain [J].
Carruthers, Vern B. ;
Suzuki, Yasuhiro .
SCHIZOPHRENIA BULLETIN, 2007, 33 (03) :745-751
[5]   Genotyping of Toxoplasma gondii isolates from cats in different geographic regions of China [J].
Chen, Z. W. ;
Gao, J. M. ;
Huo, X. X. ;
Wang, L. ;
Yu, L. ;
Halm-Lai, F. ;
Xu, Y. H. ;
Song, W. J. ;
Hide, G. ;
Shen, J. L. ;
Lun, Z. R. .
VETERINARY PARASITOLOGY, 2011, 183 (1-2) :166-170
[6]  
Coordinating Office of the National Survey on the Important Human Parasitic Diseases, 2005, Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi, V23, P332
[7]   SEPARATION OF ISOSPORA (TOXOPLASMA) GONDII CYSTS AND CYSTOZOITES FROM MOUSE-BRAIN TISSUE BY CONTINUOUS DENSITY-GRADIENT CENTRIFUGATION [J].
CORNELISSEN, AWCA ;
OVERDULVE, JP ;
HOENDERBOOM, JM .
PARASITOLOGY, 1981, 83 (AUG) :103-&
[8]   TOXOPLASMA-GONDII - BLOOD AND TISSUE KINETICS DURING ACUTE AND CHRONIC INFECTIONS IN MICE [J].
DEROUIN, F ;
GARIN, YJF .
EXPERIMENTAL PARASITOLOGY, 1991, 73 (04) :460-468
[9]   High prevalence and abundant atypical genotypes of Toxoplasma gondii isolated from lambs destined for human consumption in the USA [J].
Dubey, J. P. ;
Sundar, N. ;
Hill, D. ;
Velmurugan, G. V. ;
Bandini, L. A. ;
Kwok, O. C. H. ;
Majumdar, D. ;
Su, C. .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2008, 38 (8-9) :999-1006
[10]   Genetic characterization of Toxoplasma gondii isolates in dogs from Vietnam suggests their South American origin [J].
Dubey, J. P. ;
Huong, Lam Thi Thu ;
Sundar, N. ;
Su, C. .
VETERINARY PARASITOLOGY, 2007, 146 (3-4) :347-351