Transcriptomic analysis of gingival mesenchymal stem cells cultured on 3D bioprinted scaffold: A promising strategy for neuroregeneration

被引:44
作者
Gugliandolo, Agnese [1 ]
Diomede, Francesca [2 ]
Cardelli, Paolo [2 ]
Bramanti, Alessia [1 ,3 ]
Scionti, Domenico [1 ]
Bramanti, Placido [1 ]
Trubiani, Oriana [2 ]
Mazzon, Emanuela [1 ]
机构
[1] IRCCS Ctr Neurolesi Bonino Pulejo, Via Prov Palermo, I-98124 Messina, Italy
[2] Univ G Annunzio Chieti Pescara, Dept Med Oral & Biotechnol Sci, Stem Cells & Regenerat Med Lab, Via Vestini 31, I-66100 Chieti, Italy
[3] Natl Res Council Italy, Inst Appl Sci & Intelligent Syst ISASI Eduardo Ca, Messina, Italy
关键词
stem cells; scaffold; neurogenesis; neurotrophins; regenerative medicine; NEURAL PRECURSOR CELLS; NEURITE OUTGROWTH; NEURONAL DIFFERENTIATION; TISSUE REGENERATION; PROTEIN-KINASE; AXON GUIDANCE; PC12; CELLS; IN-VITRO; SURVIVAL; PROLIFERATION;
D O I
10.1002/jbm.a.36213
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The combined approach of mesenchymal stem cells (MSCs) and scaffolds has been proposed as a potential therapeutic tool for the treatment of neurodegenerative diseases. Indeed, even if MSCs can promote neuronal regeneration, replacing lost neurons or secreting neurotrophic factors, many limitations still exist for their application in regenerative medicine, including the low survival and differentiation rate. The scaffolds, by mimicking the endogenous microenvironment, have shown to promote cell survival, proliferation, and differentiation. In this work, gingival mesenchymal stem cells (GMSCs), isolated from healthy donors, were expanded in vitro, by culturing them adherent in plastic dishes (CTR-GMSCs) or on a poly(lactic acid) scaffold (SC-GMSCs). In order to evaluate the survival and the neurogenic differentiation potential, we performed a comparative transcriptomic analysis between CTR-GMSCs and SC-GMSCs by next generation sequencing. We found that SC-GMSCs showed an increased expression of neurogenic and prosurvival genes. In particular, genes involved in neurotrophin signaling and PI3K/Akt pathways were upregulated. On the contrary, proapoptotic and negative regulator of neuronal growth genes were downregulated. Moreover, nestin and GAP-43 protein levels increased in SC-GMSCs, confirming the neurogenic commitment of these cells. In conclusion, the scaffold, providing a trophic support for MSCs, may promote GMSCs differentiation toward a neuronal phenotype and survival. (C) 2017 Wiley Periodicals, Inc.
引用
收藏
页码:126 / 137
页数:12
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