Interphase FISH analysis on histologic sections of fixed tissues for t(11;14) (q13;q32) detection in mantle cell lymphoma and t(8;14)(q24;q32) in Burkitt's lymphoma

被引：0

作者：

Belaud-Rotureau, MA ^{[1
]}

Parrens, M

Dubus, P

Turmo, M

Lacroute, G

Taine, L

Vago, P

de Mascarel, A

Merlio, JP

机构：

[1] Univ Bordeaux 2, EA Histol & Pathol Mol 2406, F-33076 Bordeaux, France

[2] Hop Haut Leveque, Anat Pathol Lab, F-33604 Pessac, France

[3] Fac Pharm Clermont Ferrand, Serv Cytogenet Med, F-63001 Clermont Ferrand, France

[4] Hop Pellegrin, Serv Genet Med, F-33076 Bordeaux, France

来源：

ANNALES DE PATHOLOGIE | 2002年 / 22卷 / 02期

关键词：

mantle cell lymphoma; Burkitt's lymphoma; FISH; t(11; 14); t(8; paraffin section;

D O I：

暂无

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

We describe an interphase FISH analysis for formol-fixed, paraffin-embedded tissue sections with commercial probes detecting the t(11;14)(q13;q32) in mantle cell lymphoma and the t(8,14)(q24;q32) in Burkitt's lymphoma. Staining of an adjacent section allowed identification of tumoral areas, The cut-off value was evaluated in reactive lymph nodes at 5% for both probes. An incomplete hybridization pattern was found in 18 to 26% of the nuclei but was always lower than the percentages of translocated cells in tumoral regions. A t(11;14) was detected in 4/4 mantle cell lymphomas and a t(8;14) in 2/3 Burkitt's lymphomas. Interphase FISH analysis of fixed-tissue sections is a reliable technique for the direct detection of lymphoma-associated translocations on routine histological material.

引用

页码：145 / 149

页数：5

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