Protective effects of a composition of Chinese herbs-Gurigumu-13 on retinal ganglion cell apoptosis in DBA/2J glaucoma mouse model

被引：2

作者：

Zhang, Qiu-Li ^{[1
]}

Wang, Wei ^{[2
]}

Jiang, Yan ^{[3
]}

Zhang, Tian-Zi ^{[1
]}

Lu, Zhan-Jun ^{[1
]}

Jiang, Ao ^{[4
]}

机构：

[1] Inner Mongolia Univ Nationalities, Affiliated Hosp, Dept Ophthalmol, Tongliao 028000, Inner Mongolia, Peoples R China

[2] Tongliao Hosp, Dept Ophthalmol, Tongliao 028000, Inner Mongolia, Peoples R China

[3] Inner Mongolia Univ Nationalities, Affiliated Hosp, Dept Neurosurg, Tongliao 028000, Inner Mongolia, Peoples R China

[4] Jilin Univ, China Japan Union Hosp, Changchun 130000, Jilin, Peoples R China

来源：

INTERNATIONAL JOURNAL OF OPHTHALMOLOGY | 2018年 / 11卷 / 03期

基金：

中国国家自然科学基金;

关键词：

glaucoma; retinal ganglion cells; oxidative stress; endoplasmic reticulum stress; ENDOPLASMIC-RETICULUM STRESS; TRABECULAR MESHWORK CELLS; OXIDATIVE STRESS; DIABETIC-RETINOPATHY; SIGMA-RECEPTOR; DAMAGE; HYPOXIA; PROTEIN; DEATH; CHOP;

D O I：

10.18240/ijo.2018.03.03

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

AIM: To explore the concrete mechanism of a Mongolian compound medicine-Gurigumu-13 (GRGM) for glaucoma treatment. METHODS: DBA/2J mice, as glaucoma models, were intragastric administrated with GRGM to study the effect of GRGM on retinal ganglion cells (RGCs). The loss of RGCs was evaluated with the number of RGCs and axons. The expression of the target protein of RGCs or mouse retinas was determined by Western blot. The relative content of malondialdehyde (MDA) was examined by ELISA assay. RESULTS: GRGM distinctly improved retina damage via increasing the number of neurons, RGCs and axons in a concentration dependent manner. Meanwhile, GRGM obviously decreased the high level of MDA and the expression of oxidative stress-related proteins in retinas of DBA/2J mice, but promoted the expression of antioxidant proteins. Additionally, GRGM also significantly inhibited the protein expression of Bip and Chop, which were markers of endoplasmic reticulum stress-induced apoptosis. CONCLUSION: GRGM have obvious protective effects on RGCs in DBA/2J mice, and increase the number of RGCs and axons via inhibiting oxidative stress and endoplasmic reticulum stress.

引用

页码：363 / 368

页数：6

共 27 条

[1] Oxidative/Nitrosative Stress and Immuno-inflammatory Pathways in Depression: Treatment Implications [J].