Preparation and characterization of lyophilized liposomes with incorporated quercetin

被引：36

作者：

Alexopoulou, E

Georgopoulos, A

Kagkadis, KA

Demetzos, C ^{[1
]}

机构：

[1] Sch Pharm, Dept Pharmaceut Technol, Athens 15771, Greece

[2] VIANEX SA, Athens 15351, Greece

来源：

JOURNAL OF LIPOSOME RESEARCH | 2006年 / 16卷 / 01期

关键词：

liposomes; flavonoids; lyophilization;

D O I：

10.1080/08982100500528594

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Liposomes composed of egg-phosphatidylcholine (EPC) incorporating quercetin (QR) were prepared by the thin-film hydration method (TFHM) and the monophase solution method (MSM). A rapid and slow freeze-drying process was applied for both laboratory and industrial scales. The purpose of this study was to compare the two methods of liposome preparation, and further determine whether the lyophilization process affects the liposome physicochemical characteristics (size, polydispersity index, and zeta-potential) and incorporation of quercetin.

引用

收藏

页码：17 / 25

页数：9

相关论文

共 19 条

[1]

Ackland ML, 2005, IN VIVO, V19, P69

[2] Effect of freezing rate on physical stability of lyophilized cationic liposomes [J].

Aso, Y ;

Yoshioka, S .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2005, 53 (03) :301-304

[3] IS VITRIFICATION SUFFICIENT TO PRESERVE LIPOSOMES DURING FREEZE-DRYING [J].

CROWE, JH ;

LESLIE, SB ;

CROWE, LM .

CRYOBIOLOGY, 1994, 31 (04) :355-366

[4] The effects of lyophilization on the stability of liposomes containing 5-FU [J].

Glavas-Dodov, M ;

Fredro-Kumbaradzi, E ;

Goracinova, K ;

Simonoska, M ;

Calis, S ;

Trajkovic-Jolevskaa, S ;

Hincal, AA .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2005, 291 (1-2) :79-86

[5] Encapsulation of naturally occurring flavonoids into liposomes: physicochemical properties and biological activity against human cancer cell lines [J].

Goniotaki, M ;

Hatziantoniou, S ;

Dimas, K ;

Wagner, M ;

Demetzos, C .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2004, 56 (10) :1217-1224

[6] HYDROLYSIS OF PARTIALLY SATURATED EGG PHOSPHATIDYLCHOLINE IN AQUEOUS LIPOSOME DISPERSIONS AND THE EFFECT OF CHOLESTEROL INCORPORATION ON HYDROLYSIS KINETICS [J].

GRIT, M ;

ZUIDAM, NJ ;

UNDERBERG, WJM ;

CROMMELIN, DJA .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 1993, 45 (06) :490-495

[7] Effects of the acyl chain composition of phosphatidylcholines on the stability of freeze-dried small liposomes in the presence of maltose [J].

Komatsu, H ;

Saito, H ;

Okada, S ;

Tanaka, M ;

Egashira, M ;

Handa, T .

CHEMISTRY AND PHYSICS OF LIPIDS, 2001, 113 (1-2) :29-39

[8]

Lasch J., 2003, P3

[9] A novel method for the preparation of liposomes: Freeze drying of monophase solutions [J].

Li, CL ;

Deng, YJ .

JOURNAL OF PHARMACEUTICAL SCIENCES, 2004, 93 (06) :1403-1414

[10] Targeting of Liposomal Flavonoid to Liver in Combating Hepatocellular Oxidative Damage [J].

Mandal, Ardhendu K. ;

Sinha, Jayanta ;

Mandal, Suparna ;

Mukhopadhyay, Sibabrata ;

Das, Nirmalendu .

DRUG DELIVERY, 2002, 9 (03) :181-185