Lower expression of genes near microRNA in C-elegans germline

被引：7
作者：
Inaoka, H
Fukuoka, Y [1 ]
Kohane, IS
机构：
[1] Tokyo Med & Dent Univ, Sch Biomed Sci, Dept Biosyst Modeling, Tokyo, Japan
[2] Tokyo Med & Dent Univ, Inst Biomat & Biomed Engn, Dept Biomed Informat, Tokyo, Japan
[3] Harvard Univ, Sch Med, Childrens Hosp, Informat Program, Boston, MA USA
[4] Harvard Univ, Div Hlth Sci & Technol, Cambridge, MA 02138 USA
[5] MIT, Cambridge, MA 02139 USA
[6] Harvard Partners Ctr Genet & Genom, Boston, MA USA
来源：
BMC BIOINFORMATICS | 2006年 / 7卷 / 1期
关键词：
D O I：
10.1186/1471-2105-7-112
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Background: MicroRNAs ( miRNAs) are recently discovered short non-protein-coding RNA molecules. miRNAs are increasingly implicated in tissue-specific transcriptional control and particularly in development. Because there is mounting evidence for the localized component of transcriptional control, we investigated if there is a distance-dependent effect of miRNA. Results: We analyzed gene expression levels around the 84 of 113 know miRNAs for which there are nearby gene that were measured in the data in two independent C. elegans expression data sets. The expression levels are lower for genes in the vicinity of 59 of 84 (71%) miRNAs as compared to genes far from such miRNAs. Analysis of the genes with lower expression in proximity to the miRNAs reveals increased frequency matching of the 7 nucleotide "seed"s of these miRNAs. Conclusion: We found decreased messenger RNA ( mRNA) abundance, localized within a 10 kb of chromosomal distance of some miRNAs, in C. elegans germline. The increased frequency of seed matching near miRNA can explain, in part, the localized effects.
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页数：8
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