A rapid LC-MS/MS method for determination of urinary EtG and application to a cut-off limit study

被引:1
|
作者
Anilanmert, Beril [1 ]
Ozdemir, Ali Acar [3 ]
Aydin, Muhammet [2 ]
Akgul, Meral [4 ]
Cengiz, Salih [1 ]
机构
[1] Istanbul Univ, Fac Med, Inst Forens Sci, TR-34303 Cerrahpasa, Turkey
[2] Istanbul Univ, Fac Med, Dept Microbiol, TR-34303 Cerrahpasa, Turkey
[3] World Med Pharmaceut Co, TR-34212 Gunesli Istanbul, Turkey
[4] Bagcilar 6th Family Healthcare Ctr, TR-34203 Bagcilar, Turkey
关键词
ethyl glucuronide; LC-MS/MS; rapid determination in urine; validation; cut-off limit; alcohol metabolite; TANDEM MASS-SPECTROMETRY; ETHYL-GLUCURONIDE; ALCOHOL-CONSUMPTION; FORENSIC GUIDELINES; VALIDATED METHOD; SULFATE; ETHANOL; QUANTIFICATION; ETHYLGLUCURONIDE; SAMPLES;
D O I
10.1515/chempap-2015-0173
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Ethyl glucuronide (EtG) is a metabolite and a specific marker of alcohol consumption that can be detected days after the complete elimination of alcohol after drinking. A rapid, simple, and sensitive LC-ESI-MS/MS method for the determination of urinary ethyl glucuronide was developed and fully validated in accordance with analytical standards, using the C-18 column. The whole process including sample preparation and LC-MS/MS lasted 10 min. A comprehensive validation including HorRat, measurement uncertainty, system suitability and intermediate precision calculations among analysts, and a cut-off limit study was performed. The method was applied to real samples and a cutoff limit determination study. The LOD and LOQ (using the IUPAC and Eurachem methods) were determined as 104.21 ng mL(-1) and 165.00 ng mL(-1). A cut-off limit of approximate to 818 ng mg(-1) (normalised to creatinine) was found for urinary EtG. The results showed that the cut-off limits currently in use should be re-considered in further studies and standardised on a global scale. Normalisation to creatinine is important because of the risk of the dilution of urine intentionally or with a change of diet. The concentrations of real samples from subjects who had consumed alcohol were successfully predicted using this method, after zero HS-GC/MS results of urine alcohol concentration. (C) 2015 Institute of Chemistry, Slovak Academy of Sciences
引用
收藏
页码:1532 / 1540
页数:9
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