Repeat UVA exposure of human skin fibroblasts induces both a transitionary and recovery DNA methylation response

被引：4
作者：
Tilburg, Julia [1 ,2 ]
Slieker, Roderick C. [1 ]
Suchiman, H. Eka D. [1 ]
Heath, Alan [3 ]
van Heemst, Diana [4 ]
Slagboom, P. Eline [1 ]
de Gruijl, Frank R. [5 ]
Gunn, David A. [3 ]
Heijmans, Bastiaan T. [1 ]
机构：
[1] Leiden Univ Med Ctr, Dept Biomed Data Sci, Mol Epidemiol, Leiden, Netherlands
[2] Leiden Univ Med Ctr, Div Thrombosis & Hemostasis, Dept Internal Med, Einthoven Lab Expt Vasc Med, Leiden, Netherlands
[3] Unilever Res Labs, Colworth Sci Pk, Sharnbrook, Beds, England
[4] Leiden Univ Med Ctr, Dept Internal Med, Gerontol & Geriatr, Leiden, Netherlands
[5] Leiden Univ Med Ctr, Dept Dermatol, Leiden, Netherlands
来源：
EPIGENOMICS | 2020年 / 12卷 / 07期
关键词：
dermis; fibroblasts; genome-wide DNA methylation; photoaging; skin aging; UVA irradiation; DIMERS; DAMAGE; ASSOCIATION; PHOTODAMAGE; RADIATION; CAPACITY; AGE;
D O I：
10.2217/epi-2019-0251
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Aim: UVA radiation drives skin photoaging in the dermis, plausibly via persistent changes to DNA methylation in dermal fibroblasts. Methods: Genome-wide DNA methylation changes after five repeated daily UVA doses were determined at 48 h (transitionary) and 1 week (recovery) post final irradiation. Results: Differential methylation was found at the transitionary time point in active chromatin states near genes that are highly expressed in fibroblasts and are involved in cellular defensive mechanisms; the majority of these methylation differences were restored to control levels after 7 day recovery. At the recovery time point, new differential methylation occurred at repressed regions near developmental genes, normally weakly expressed in fibroblasts. Conclusion: UVA irradiation induces transitionary and recovery-associated DNA methylation responses in fibroblasts with contrasting functional characteristics.
引用
收藏
页码：563 / 573
页数：11
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