Effects of perinatal di (2-ethylhexyl) phthalate exposure on thyroid function in rat offspring

被引：42

作者：

Dong, Jing ^{[1
]}

Cong, Zhangzhao ^{[1
]}

You, Mingdan ^{[1
]}

Fu, Yuanyuan ^{[1
]}

Wang, Yi ^{[1
]}

Wang, Yuan ^{[1
]}

Fu, Hui ^{[1
]}

Wei, Lingling ^{[1
]}

Chen, Jie ^{[1
]}

机构：

[1] China Med Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth, 77 Puhe Rd, Shenyang 110122, Liaoning, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2019年 / 67卷

基金：

中国国家自然科学基金;

关键词：

DEHP; Perinatal period; Thyroid function; Endocrine disruptors; Offspring; DI(2-ETHYLHEXYL)PHTHALATE DEHP; ENDOCRINE DISRUPTORS; CELL-PROLIFERATION; NA+/I-SYMPORTER; FACTOR-I; GENE; PAX-8; TTF-1; TRANSCRIPTION; DEIODINASES;

D O I：

10.1016/j.etap.2019.01.012

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Di (2-ethylhexyl) phthalate (DEHP) is a commonly used plasticizer in industry and displays the characteristics of an endocrine disruptor. Disorders of the maternal thyroid hormone (TH) during pregnancy can cause adverse effects on the fetus. We investigated the effects and possible mechanism of perinatal DEHP exposure on the thyroid function of pups. Pregnant female Wistar rats were randomly divided into four groups and received doses of DEHP of 0, 30, 300, 750 mg/kg/day by gavage at from gestational day (GD) 0 to postnatal day (PN) 21. The concentration of serum THs and the ultrastructure of thyroid follicular cells in the offspring were examined. Related protein level and gene expression of thyroid proteins in pups were analyzed by western blotting and real-ltime PCR. We found that DEHP significantly reduced total thyroxine (TT4) and increased thyroid stimulating hormone (TSH) in pups, while total triiodothyronine (TT3) showed no change. Thyroid follicular cells ultra structure was damaged in DEHP exposed pups as viewed by electron microscopy. Furthermore, exposure to DEHP significantly increased protein and mRNA levels of thyroid transcription factor 1 (TTF-1), paired box 8 (PAX8), sodium iodide symporter (NIS) and thyroid peroxidase (TPO) in pups. In addition, levels of deiodinases of pups were also affected. These findings indicated that DEHP can disrupt thyroid function by damaging thyroid follicles and affecting TTF-1, PAX8, NIS, TPO and the deiodinase protein family.

引用

页码：53 / 60

页数：8

共 75 条

[1] Mechanism of diethylhexylphthalate (DEHP) induced testicular damage and of grape seed extract-induced protection in the rat [J].

Abdel-Kawi, Samraa H. ;

Hashem, Khalid S. ;

Abd-Allah, Saber .

FOOD AND CHEMICAL TOXICOLOGY, 2016, 90 :64-75

[2]

Altmann A, 2005, J NUCL MED, V46, P831

[3]

Ambrosio R., 2017, MOL CELL ENDOCRINOL

[4] Association between Maternal Exposure to di(2-ethylhexyl) Phthalate and Reproductive Hormone Levels in Fetal Blood: The Hokkaido Study on Environment and Children's Health [J].

Araki, Atsuko ;

Mitsui, Takahiko ;

Miyashita, Chihiro ;

Nakajima, Tamie ;

Naito, Hisao ;

Ito, Sachiko ;

Sasaki, Seiko ;

Cho, Kazutoshi ;

Ikeno, Tamiko ;

Nonomura, Katsuya ;

Kishi, Reiko .

PLOS ONE, 2014, 9 (10)

[5] Maternal and early life exposure to phthalates: The Plastics and Personal-care Products use in Pregnancy (P4) study [J].

Arbuckle, Tye E. ;

Fisher, Mandy ;

MacPherson, Susan ;

Lang, Carly ;

Provencher, Gilles ;

LeBlanc, Alain ;

Hauser, Russ ;

Feeley, Mark ;

Ayotte, Pierre ;

Neisa, Angelica ;

Ramsay, Tim ;

Tawagi, George .

SCIENCE OF THE TOTAL ENVIRONMENT, 2016, 551 :344-356

[6] Critical role of the hypothalamic-pituitary-thyroid axis in bone [J].

Bassett, J. H. Duncan ;

Williams, Graham R. .

BONE, 2008, 43 (03) :418-426

[7] Thyroid Function Within the Normal Range, Subclinical Hypothyroidism, and the Risk of Atrial Fibrillation [J].

Baumgartner, Christine ;

da Costa, Bruno R. ;

Collet, Tinh-Hai ;

Feller, Martin ;

Floriani, Carmen ;

Bauer, Douglas C. ;

Cappola, Anne R. ;

Heckbert, Susan R. ;

Ceresini, Graziano ;

Gussekloo, Jacobijn ;

den Elzen, Wendy P. J. ;

Peeters, Robin P. ;

Luben, Robert ;

Voelzke, Henry ;

Doerr, Marcus ;

Walsh, John P. ;

Bremner, Alexandra ;

Iacoviello, Massimo ;

Macfarlane, Peter ;

Heeringa, Jan ;

Stott, David J. ;

Westendorp, Rudi G. J. ;

Khaw, Kay-Tee ;

Magnani, Jared W. ;

Aujesky, Drahomir ;

Rodondi, Nicolas .

CIRCULATION, 2017, 136 (22) :2100-2116

[8] Childhood Exposure to Phthalates: Associations with Thyroid Function, Insulin-like Growth Factor I, and Growth [J].

Boas, Malene ;

Frederiksen, Hanne ;

Feldt-Rasmussen, Ulla ;

Skakkebaek, Niels E. ;

Hegedus, Laszlo ;

Hilsted, Linda ;

Juul, Anders ;

Main, Katharina M. .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2010, 118 (10) :1458-1464

[9] Thyroid transcription factor-1 (TTF-1/Nkx2.1/TITF1) gene regulation in the lung [J].

Boggaram, Vijay .

CLINICAL SCIENCE, 2009, 116 (1-2) :27-35

[10] Effects of environmental synthetic chemicals on thyroid function [J].

Brucker-Davis, F .

THYROID, 1998, 8 (09) :827-856

← 1 2 3 4 5 6 7 8 →