Population genetic analysis reveals a low level of genetic diversity of 'Candidatus Phytoplasma aurantifolia' causing witches' broom disease in lime

被引：13

作者：

Al-Abadi, Shaikha Y. ^{[1
]}

Al-Sadi, Abdullah M. ^{[2
]}

Dickinson, Matthew ^{[3
]}

Al-Hammadi, Mohammed S. ^{[4
]}

Al-Shariqi, Rashid ^{[4
]}

Al-Yahyai, Rashid A. ^{[2
]}

Kazerooni, Elham A. ^{[5
]}

Bertaccini, Assunta ^{[6
]}

机构：

[1] Royal Court Affairs, Seeb, Oman

[2] Sultan Qaboos Univ, Coll Agr & Marine Sci, Dept Crop Sci, POB 8, Al Khoud 123, Oman

[3] Univ Nottingham, Sch Biosci, Sutton Bonington Campus, Loughborough, Leics, England

[4] Abu Dhabi Food Control Author, POB 52150, Abu Dhabi, U Arab Emirates

[5] Islamic Azad Univ, Shiraz, Iran

[6] Univ Bologna, Alma Mater Studiorum, Dept Agr Sci, Plant Pathol, Bologna, Italy

来源：

SPRINGERPLUS | 2016年 / 5卷

关键词：

WBDL; Phytoplasma; Acid lime; Population structure; ACID LIME; CITRUS; OMAN;

D O I：

10.1186/s40064-016-3401-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Witches' broom disease of lime (WBDL) is a serious phytoplasma disease of acid lime in Oman, the UAE and Iran. Despite efforts to study it, no systemic study attempted to characterize the relationship among the associated phytoplasma, 'Candidatus Phytoplasma aurantifolia', from the three countries. This study utilized sequences of the 16S rRNA, imp and secA genes to characterize 57 strains collected from Oman (38), the UAE (9) and Iran (10). Phylogenetic analysis based on the 16S rRNA gene showed that the 57 strains shared 98.5-100 % nucleotide similarity to each other and to strains of 'Ca. P. aurantifolia' available in GenBank. The level of genetic diversity was low based on the 16S rRNA (0-0.011), imp (0-0.002) and secA genes (0-0.015). The presence of low level of diversity among phytoplasma strains from Oman, the UAE and Iran can be explained by the movement of infected lime seedlings from one country to another through trading and exchange of infected plants. The study discusses implication of the findings on WBDL spread and management.

引用

页数：11

共 21 条

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