EBF1 Negatively Regulates Brassinosteroid-Induced Apical Hook Development and Cell Elongation through Promoting BZR1 Degradation

被引:7
|
作者
Zhao, Na [1 ]
Zhao, Min [1 ]
Wang, Lingyan [1 ]
Han, Chao [1 ]
Bai, Mingyi [1 ]
Fan, Min [1 ]
机构
[1] Shandong Univ, Minist Educ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Qingdao 266237, Peoples R China
基金
中国国家自然科学基金;
关键词
brassinosteroid; EBF1; BZR1; EIN3; PIF4; F-BOX PROTEINS; ETHYLENE; GENE; GIBBERELLIN; TARGETS; GROWTH; PIF3;
D O I
10.3390/ijms232415889
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Brassinosteroids (BRs) are a group of plant steroid hormones that play important roles in a wide range of developmental and physiological processes in plants. Transcription factors BRASSINOZALE-RESISTANT1 (BZR1) and its homologs are key components of BR signaling and integrate a wide range of internal and environmental signals to coordinate plant growth and development. Although several E3 ligases have been reported to regulate the stability of BZR1, the molecular mechanism of BZR1 degradation remains unclear. Here, we reveal how a newly identified molecular mechanism underlying EBF1 directly regulates BZR1 protein stability via the 26S proteasome pathway, repressing BR function on regulating Arabidopsis apical hook development and hypocotyl elongation. BZR1 directly binds to the EBF1 gene promotor to reduce EBF1 expression. Furthermore, the genetic analysis shows that BZR1, EIN3 and PIF4 interdependently regulate plant apical hook development. Taken together, our data demonstrates that EBF1 is a negative regulator of the BR signaling pathway.
引用
收藏
页数:14
相关论文
共 32 条
  • [1] TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1
    Oh, Eunkyoo
    Zhu, Jia-Ying
    Ryu, Hojin
    Hwang, Ildoo
    Wang, Zhi-Yong
    NATURE COMMUNICATIONS, 2014, 5
  • [2] TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1
    Eunkyoo Oh
    Jia-Ying Zhu
    Hojin Ryu
    Ildoo Hwang
    Zhi-Yong Wang
    Nature Communications, 5
  • [3] BZR1 Mediates Brassinosteroid-Induced Autophagy and Nitrogen Starvation in Tomato
    Wang, Yu
    Cao, Jia-Jian
    Wang, Kai-Xin
    Xia, Xiao-Jian
    Shi, Kai
    Zhou, Yan-Hong
    Yu, Jing-Quan
    Zhou, Jie
    PLANT PHYSIOLOGY, 2019, 179 (02) : 671 - 685
  • [4] Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis
    Wang, ZY
    Nakano, T
    Gendron, J
    He, JX
    Chen, M
    Vafeados, D
    Yang, YL
    Fujioka, S
    Yoshida, S
    Asami, T
    Chory, J
    DEVELOPMENTAL CELL, 2002, 2 (04) : 505 - 513
  • [5] BZR1 targets steroid 22-alpha hydroxylase 4 to negatively regulates cell elongation in bamboo
    Wang, Sining
    Lou, Yongfeng
    Qi, Haole
    Li, Hui
    Di, Xiaolin
    Sun, Huayu
    Gao, Zhimin
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2025, 289
  • [6] Brassinosteroid-induced gene repression requires specific and tight promoter binding of BIL1/BZR1 via DNA shape readout
    Shohei Nosaki
    Nobutaka Mitsuda
    Shingo Sakamoto
    Kazuki Kusubayashi
    Ayumi Yamagami
    Yuqun Xu
    Thi Bao Chau Bui
    Tohru Terada
    Kenji Miura
    Takeshi Nakano
    Masaru Tanokura
    Takuya Miyakawa
    Nature Plants, 2022, 8 : 1440 - 1452
  • [7] Brassinosteroid regulates stomatal development in etiolated Arabidopsis cotyledons via transcription factors BZR1 and BES1
    Li, Shuo
    Yan, Jin
    Chen, Lian-Ge
    Meng, Guanghua
    Zhou, Yuling
    Wang, Chun-Ming
    Jiang, Lei
    Luo, Juan
    Jiang, Yueming
    Li, Qian-Feng
    Tang, Wenqiang
    He, Jun-Xian
    PLANT PHYSIOLOGY, 2024, 195 (02) : 1382 - 1400
  • [8] Brassinosteroid-induced gene repression requires specific and tight promoter binding of BIL1/BZR1 via DNA shape readout
    Nosaki, Shohei
    Mitsuda, Nobutaka
    Sakamoto, Shingo
    Kusubayashi, Kazuki
    Yamagami, Ayumi
    Xu, Yuqun
    Bui, Thi Bao Chau
    Terada, Tohru
    Miura, Kenji
    Nakano, Takeshi
    Tanokura, Masaru
    Miyakawa, Takuya
    NATURE PLANTS, 2022, 8 (12) : 1440 - 1452
  • [9] ATBS1-INTERACTING FACTOR 2 negatively regulates dark- and brassinosteroid-induced leaf senescence through interactions with INDUCER OF CBF EXPRESSION 1
    Kim, Yoon
    Park, Seon-U
    Shin, Dong-Min
    Giang Pham
    Jeong, You Seung
    Kim, Soo-Hwan
    JOURNAL OF EXPERIMENTAL BOTANY, 2020, 71 (04) : 1475 - 1490
  • [10] ZFP521 transcription factor modulates B cell development through interactions with EBF1
    Lei, Hong
    Lukin, Kara
    Warming, Soren
    Hagman, James
    JOURNAL OF IMMUNOLOGY, 2013, 190