A canine cancer-gene microarray for CGH analysis of tumors

被引：24

作者：

Thomas, R

Fiegler, H

Ostrander, EA

Galibert, F

Carter, NP

Breen, M

机构：

[1] N Carolina State Univ, Coll Vet Med, Dept Mol Biomed Sci, Raleigh, NC 27606 USA

[2] Anim Hlth Trust, Ctr Prevent Med, Oncol Res Grp, Newmarket, Suffolk, England

[3] Wellcome Trust Sanger Inst, Cambridge, England

[4] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98104 USA

[5] Fred Hutchinson Canc Res Ctr, Human Biol Div, Seattle, WA 98104 USA

[6] Fac Med, UMR 6061 CNRS, Rennes, France

来源：

CYTOGENETIC AND GENOME RESEARCH | 2003年 / 102卷 / 1-4期

关键词：

D O I：

10.1159/000075758

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

As with many human cancers, canine tumors demonstrate recurrent chromosome aberrations. A detailed knowledge of such aberrations may facilitate diagnosis, prognosis and the selection of appropriate therapy. Following recent advances made in human genomics, we are developing a DNA microarray for the domestic dog, to be used in the detection and characterization of copy number changes in canine tumors. As a proof of principle, we have developed a small-scale microarray comprising 87 canine BAC clones. The array is composed of 26 clones selected from a panel of 24 canine cancer genes, representing 18 chromosomes, and an additional set of clones representing dog chromosomes 11, 13, 14 and 31. These chromosomes were shown previously to be commonly aberrant in canine multicentric malignant lymphoma. Clones representing the sex chromosomes were also included. We outline the principles of canine microarray development, and present data obtained from microarray analysis of three canine lymphoma cases previously characterized using conventional cytogenetic techniques. Copyright (C) 2003 S. Karger AG, Basel.

引用

页码：254 / 260

页数：7

共 21 条

[11] Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library [J].

Li, R ;

Mignot, E ;

Faraco, J ;

Kadotani, H ;

Cantanese, J ;

Zhao, B ;

Lin, X ;

Hinton, L ;

Ostrander, EA ;

Patterson, DF ;

de Jong, PJ .

GENOMICS, 1999, 58 (01) :9-17

[12] New tools in molecular pathology - Commentary [J].

Lichter, P .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2000, 2 (04) :171-173

[13] High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays [J].

Pinkel, D ;

Seagraves, R ;

Sudar, D ;

Clark, S ;

Poole, I ;

Kowbel, D ;

Collins, C ;

Kuo, WL ;

Chen, C ;

Zhai, Y ;

Dairkee, SH ;

Ljung, BM ;

Gray, JW ;

Albertson, DG .

NATURE GENETICS, 1998, 20 (02) :207-211

[14] Assembly of microarrays for genome-wide measurement of DNA copy number. [J].

Snijders, AM ;

Nowak, N ;

Segraves, R ;

Blackwood, S ;

Brown, N ;

Conroy, J ;

Hamilton, G ;

Hindle, AK ;

Huey, B ;

Kimura, K ;

Law, S ;

Myambo, K ;

Palmer, J ;

Ylstra, B ;

Yue, JP ;

Gray, JW ;

Jain, AN ;

Pinkel, D ;

Albertson, DG .

NATURE GENETICS, 2001, 29 (03) :263-264

[15]

SolinasToldo S, 1997, GENE CHROMOSOME CANC, V20, P399, DOI 10.1002/(SICI)1098-2264(199712)20:4<399::AID-GCC12>3.0.CO

[16]

2-I

[17]

SOLINASTOLDO S, 1993, HUM MOL GENET, V2, P1907

[18]

SPEICHER MR, 1999, CYTOGENET CELL GENET, V87, P4

[19] Isolation and chromosomal assignment of canine genomic BAC clones representing 25 cancer-related genes [J].

Thomas, R ;

Bridge, W ;

Benke, K ;

Breen, M .

CYTOGENETIC AND GENOME RESEARCH, 2003, 102 (1-4) :249-253

[20] Chromosome aberrations in canine multicentric lymphomas detected with comparative genomic hybridisation and a panel of single locus probes [J].

Thomas, R ;

Smith, KC ;

Ostrander, EA ;

Galibert, F ;

Breen, M .

BRITISH JOURNAL OF CANCER, 2003, 89 (08) :1530-1537

← 1 2 3 →