A canine cancer-gene microarray for CGH analysis of tumors

被引：24

作者：

Thomas, R

Fiegler, H

Ostrander, EA

Galibert, F

Carter, NP

Breen, M

机构：

[1] N Carolina State Univ, Coll Vet Med, Dept Mol Biomed Sci, Raleigh, NC 27606 USA

[2] Anim Hlth Trust, Ctr Prevent Med, Oncol Res Grp, Newmarket, Suffolk, England

[3] Wellcome Trust Sanger Inst, Cambridge, England

[4] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98104 USA

[5] Fred Hutchinson Canc Res Ctr, Human Biol Div, Seattle, WA 98104 USA

[6] Fac Med, UMR 6061 CNRS, Rennes, France

来源：

CYTOGENETIC AND GENOME RESEARCH | 2003年 / 102卷 / 1-4期

关键词：

D O I：

10.1159/000075758

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

As with many human cancers, canine tumors demonstrate recurrent chromosome aberrations. A detailed knowledge of such aberrations may facilitate diagnosis, prognosis and the selection of appropriate therapy. Following recent advances made in human genomics, we are developing a DNA microarray for the domestic dog, to be used in the detection and characterization of copy number changes in canine tumors. As a proof of principle, we have developed a small-scale microarray comprising 87 canine BAC clones. The array is composed of 26 clones selected from a panel of 24 canine cancer genes, representing 18 chromosomes, and an additional set of clones representing dog chromosomes 11, 13, 14 and 31. These chromosomes were shown previously to be commonly aberrant in canine multicentric malignant lymphoma. Clones representing the sex chromosomes were also included. We outline the principles of canine microarray development, and present data obtained from microarray analysis of three canine lymphoma cases previously characterized using conventional cytogenetic techniques. Copyright (C) 2003 S. Karger AG, Basel.

引用

收藏

页码：254 / 260

页数：7

相关论文

共 21 条

[1] Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene [J].

Albertson, DG ;

Ylstra, B ;

Segraves, R ;

Collins, C ;

Dairkee, SH ;

Kowbel, D ;

Kuo, WL ;

Gray, JW ;

Pinkel, D .

NATURE GENETICS, 2000, 25 (02) :144-146

[2] Reciprocal chromosome painting reveals detailed regions of conserved synteny between the karyotypes of the domestic dog (Canis familiaris) and human [J].

Breen, M ;

Thomas, R ;

Binns, MM ;

Carter, NP ;

Langford, CF .

GENOMICS, 1999, 61 (02) :145-155

[3] Chromosome-specific single-locus FISH probes allow anchorage of an 1800-marker integrated radiation-hybrid/linkage map of the domestic dog genome to all chromosomes [J].

Breen, M ;

Jouquand, S ;

Renier, C ;

Mellersh, CS ;

Hitte, C ;

Holmes, NG ;

Chéron, A ;

Suter, N ;

Vignaux, F ;

Bristow, AE ;

Priat, C ;

McCann, E ;

André, C ;

Boundy, S ;

Gitsham, P ;

Thomas, R ;

Bridge, WL ;

Spriggs, HF ;

Ryder, EJ ;

Curson, A ;

Sampson, J ;

Ostrander, EA ;

Binns, MM ;

Galibert, F .

GENOME RESEARCH, 2001, 11 (10) :1784-1795

[4]

Breen M, 1999, CHROMOSOME RES, V7, P575

[5] Comparative genomic hybridization (CGH) in dogs - Application to the study of a canine glial tumour cell line [J].

Dunn, KA ;

Thomas, R ;

Binns, MM ;

Breen, M .

VETERINARY JOURNAL, 2000, 160 (01) :77-82

[6] DNA microarrays for comparative genomic hybridization based on DOP-PCR amplification of BAC and PAC clones [J].

Fiegler, H ;

Carr, P ;

Douglas, EJ ;

Burford, DC ;

Hunt, S ;

Smith, J ;

Vetrie, D ;

Gorman, P ;

Tomlinson, IPM ;

Carter, NP .

GENES CHROMOSOMES & CANCER, 2003, 36 (04) :361-374

[7] A 1-Mb resolution radiation hybrid map of the canine genome [J].

Guyon, R ;

Lorentzen, TD ;

Hitte, C ;

Kim, L ;

Cadieu, E ;

Parker, HG ;

Quignon, P ;

Lowe, JK ;

Renier, C ;

Gelfenbeyn, B ;

Vignaux, F ;

DeFrance, HB ;

Gloux, S ;

Mahairas, GG ;

André, C ;

Galiber, F ;

Ostrander, EA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (09) :5296-5301

[8] Genome scanning with array CGH delineates regional alterations in mouse islet carcinomas [J].

Hodgson G. ;

Hager J.H. ;

Volik S. ;

Hariono S. ;

Wernick M. ;

Moore D. ;

Albertson D.G. ;

Pinkel D. ;

Collins C. ;

Hanahan D. ;

Gray J.W. .

Nature Genetics, 2001, 29 (4) :459-464

[9] COMPARATIVE GENOMIC HYBRIDIZATION FOR MOLECULAR CYTOGENETIC ANALYSIS OF SOLID TUMORS [J].

KALLIONIEMI, A ;

KALLIONIEMI, OP ;

SUDAR, D ;

RUTOVITZ, D ;

GRAY, JW ;

WALDMAN, F ;

PINKEL, D .

SCIENCE, 1992, 258 (5083) :818-821

[10] High resolution comparative genomic hybridisation in clinical cytogenetics [J].

Kirchhoff, M ;

Rose, H ;

Lundsteen, C .

JOURNAL OF MEDICAL GENETICS, 2001, 38 (11) :740-744