A novel link between angiogenesis and natural products: Anti-angiogenic effects of Opuntia dillenii

被引:5
作者
Ejaz, Sohail [1 ]
Anwar, Khaleeq [2 ]
Taj, Robina [2 ]
Ashraf, Muhammad [2 ]
机构
[1] Univ Cambridge, Addenbrookes Hosp, Stroke Res Grp, Dept Clin Neurosci, Cambridge CB2 2QQ, England
[2] Univ Vet & Anim Sci, Dept Pharmacol & Toxicol, Angiogenesis & Toxicol Res Lab, Lahore 54000, Pakistan
来源
CENTRAL EUROPEAN JOURNAL OF BIOLOGY | 2014年 / 9卷 / 03期
关键词
Angiogenesis; Opuntia dillenii; CAM assay; Phyto chemicals; CACTUS PEAR; CANCER; POLYSACCHARIDES; EXTRACTS; GROWTH; PLANTS;
D O I
10.2478/s11535-013-0266-x
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Angiogenesis is the formation of new blood vessels from the existing blood vessels and is involved in both physiological and pathological events. Pathological angiogenesis provokes in several important diseases as in inflammatory diseases, ischemic conditions, diabetic neuropathy and cancer. The discovery of new drugs from phyto-chemicals has a long history and anti-angiogenic agents from plant sources provide a platform for the development of phyto-medicines to treat/control pathological angiogenesis. The current project was designed to determine the efficacy of different concentrations of aqueous extract from Opuntia dillenii (OD) on angiogenesis by employing chicken chorioallantoic membrane (CAM) assay. Computer based 3D image probing was utilized to quantify anti-angiogenic effects of OD extract. Extremely aggravated dose dependent anti-angiogenic response was recorded in all groups treated with OD extract with significant reduction (P < 0.01) in total area, diameter of the primary, secondary, tertiary blood vessels and capillary plexuses. Analysis of 3D parameters of OD treated CAMs revealed deteriorated angular spectrum and reduction in the height of Abbott curves. In addition, histological data revealed thinning of ectodermal layer and damaged extracellular matrix. The results spotlight that OD extract possess considerable anti-angiogenic potential and further characterization/isolation of diverse phyto-chemicals would turn up with the discovery of novel phyto-agents to control/mitigate pathological angiogenesis.
引用
收藏
页码:298 / 308
页数:11
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