Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber

被引：53

作者：

Breunig, Hans Georg ^{[1
,2
]}

Weinigel, Martin ^{[1
]}

Bueckle, Rainer ^{[1
]}

Kellner-Hoefer, Marcel ^{[1
]}

Lademann, Juergen ^{[3
]}

Darvin, Maxim E. ^{[3
]}

Sterry, Wolfram ^{[3
]}

Koenig, Karsten ^{[1
,2
]}

机构：

[1] JenLab GmbH, D-07745 Jena, Germany

[2] Univ Saarland, Dept Biophoton & Laser Technol, D-66123 Saarbrucken, Germany

[3] Charite, Dept Dermatol Venerol & Allergol, D-10117 Berlin, Germany

来源：

LASER PHYSICS LETTERS | 2013年 / 10卷 / 02期

关键词：

NONLINEAR-OPTICAL MICROSCOPY; HIGH-SPECTRAL-RESOLUTION; IN-VIVO; SPECTROSCOPY; FLUORESCENCE;

D O I：

10.1088/1612-2011/10/2/025604

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on in vivo coherent anti-Stokes Raman scattering spectroscopy (CARS), two-photon fluorescence and second-harmonic-generation imaging on human skin with a novel multimodal clinical CARS/multiphoton tomograph. CARS imaging is realized by a combination of femtosecond pulses with broadband continuum pulses generated by a photonic crystal fiber. The images reveal the microscopic distribution of (i) non-fluorescent lipids, (ii) endogenous fluorophores and (iii) the collagen network inside the human skin in vivo with subcellular resolution. Examples of healthy as well as cancer-affected skin are presented.

引用

页数：5

共 31 条

[21] High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution [J].

König, K ;

Riemann, I .

JOURNAL OF BIOMEDICAL OPTICS, 2003, 8 (03) :432-439

[22] A comparative Raman and CARS imaging study of colon tissue [J].

Krafft, Christoph ;

Ramoji, Anuradha A. ;

Bielecki, Christiane ;

Vogler, Nadine ;

Meyer, Tobias ;

Akimov, Denis ;

Roesch, Petra ;

Schmitt, Michael ;

Dietzek, Benjamin ;

Petersen, Iver ;

Stallmach, Andreas ;

Popp, Juergen .

JOURNAL OF BIOPHOTONICS, 2009, 2 (05) :303-312

[23]

Masters B.R., 2008, Handbook of biomedical nonlinear optical microscopy

[24] Coherent anti-Stokes Raman scattering microscopy using photonic crystal fiber with two closely lying zero dispersion wavelengths [J].

Murugkar, Sangeeta ;

Brideau, Craig ;

Ridsdale, Andrew ;

Naji, Majid ;

Stys, Peter K. ;

Anis, Hanan .

OPTICS EXPRESS, 2007, 15 (21) :14028-14037

[25] Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source [J].

Paulsen, HN ;

Hilligsoe, KM ;

Thogersen, J ;

Keiding, SR ;

Larsen, JJ .

OPTICS LETTERS, 2003, 28 (13) :1123-1125

[26] The need for speed [J].

Suhalim, Jeffrey L. ;

Boik, John C. ;

Tromberg, Bruce J. ;

Potma, Eric O. .

JOURNAL OF BIOPHOTONICS, 2012, 5 (5-6) :387-395

[27] Towards multimodal nonlinear optical tomography - experimental methodology [J].

Vogler, N. ;

Medyukhina, A. ;

Latka, I. ;

Kemper, S. ;

Beohm, M. ;

Dietzek, B. ;

Popp, J. .

LASER PHYSICS LETTERS, 2011, 8 (08) :617-624

[28] Two-color Raman spectroscopy for the simultaneous detection of chemotherapeutics and antioxidative status of human skin [J].

Werncke, W. ;

Latka, I. ;

Sassning, S. ;

Dietzek, B. ;

Darvin, M. E. ;

Meinke, M. C. ;

Popp, J. ;

Koenig, K. ;

Fluhr, J. W. ;

Lademann, J. .

LASER PHYSICS LETTERS, 2011, 8 (12) :895-900

[29] CARS module for multimodal microscopy [J].

Zadoyan, Ruben ;

Baldacchini, Tommaso ;

Carter, John ;

Kuo, Chun-Hung ;

Ocepek, David .

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XI, 2011, 7903

[30] Nonlinear magic: multiphoton microscopy in the biosciences [J].

Zipfel, WR ;

Williams, RM ;

Webb, WW .

NATURE BIOTECHNOLOGY, 2003, 21 (11) :1368-1376

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