Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber

被引：53

作者：

Breunig, Hans Georg ^{[1
,2
]}

Weinigel, Martin ^{[1
]}

Bueckle, Rainer ^{[1
]}

Kellner-Hoefer, Marcel ^{[1
]}

Lademann, Juergen ^{[3
]}

Darvin, Maxim E. ^{[3
]}

Sterry, Wolfram ^{[3
]}

Koenig, Karsten ^{[1
,2
]}

机构：

[1] JenLab GmbH, D-07745 Jena, Germany

[2] Univ Saarland, Dept Biophoton & Laser Technol, D-66123 Saarbrucken, Germany

[3] Charite, Dept Dermatol Venerol & Allergol, D-10117 Berlin, Germany

来源：

LASER PHYSICS LETTERS | 2013年 / 10卷 / 02期

关键词：

NONLINEAR-OPTICAL MICROSCOPY; HIGH-SPECTRAL-RESOLUTION; IN-VIVO; SPECTROSCOPY; FLUORESCENCE;

D O I：

10.1088/1612-2011/10/2/025604

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on in vivo coherent anti-Stokes Raman scattering spectroscopy (CARS), two-photon fluorescence and second-harmonic-generation imaging on human skin with a novel multimodal clinical CARS/multiphoton tomograph. CARS imaging is realized by a combination of femtosecond pulses with broadband continuum pulses generated by a photonic crystal fiber. The images reveal the microscopic distribution of (i) non-fluorescent lipids, (ii) endogenous fluorophores and (iii) the collagen network inside the human skin in vivo with subcellular resolution. Examples of healthy as well as cancer-affected skin are presented.

引用

页数：5

共 31 条

[1] Picosecond anti-Stokes generation in a photonic-crystal fiber for interferometric CARS microscopy [J].

Andresen, Esben Ravn ;

Keiding, Soren Rud ;

Potma, Eric Olaf .

OPTICS EXPRESS, 2006, 14 (16) :7246-7251

[2] Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging [J].

Andresen, Volker ;

Alexander, Stephanie ;

Heupel, Wolfgang-Moritz ;

Hirschberg, Markus ;

Hoffman, Robert M. ;

Friedl, Peter .

CURRENT OPINION IN BIOTECHNOLOGY, 2009, 20 (01) :54-62

[3] Combined in vivo multiphoton and CARS imaging of healthy and disease-affected human skin [J].

Breunig, Hans Georg ;

Bueckle, Rainer ;

Kellner-Hoefer, Marcel ;

Weinigel, Martin ;

Lademann, Juergen ;

Sterry, Wolfram ;

Koenig, Karsten .

MICROSCOPY RESEARCH AND TECHNIQUE, 2012, 75 (04) :492-498

[4] Multiphoton excitation characteristics of cellular fluorophores of human skin in vivo [J].

Breunig, Hans Georg ;

Studier, Hauke ;

Koenig, Karsten .

OPTICS EXPRESS, 2010, 18 (08) :7857-7871

[5] Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms [J].

Campagnola, PJ ;

Loew, LM .

NATURE BIOTECHNOLOGY, 2003, 21 (11) :1356-1360

[6] A multimodal platform for nonlinear optical microscopy and microspectroscopy [J].

Chen, Hongtao ;

Wang, Haifeng ;

Slipchenko, Mikhail N. ;

Jung, YooKyung ;

Shi, Yunzhou ;

Zhu, Jiabin ;

Buhman, Kimberly K. ;

Cheng, Ji-Xin .

OPTICS EXPRESS, 2009, 17 (03) :1282-1290

[7] Coherent anti-Stokes Raman scattering microscopy: Instrumentation, theory, and applications [J].

Cheng, JX ;

Xie, XS .

JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (03) :827-840

[8] An epi-detected coherent anti-stokes raman scattering (E-CARS) microscope with high spectral resolution and high sensitivity [J].

Cheng, JX ;

Volkmer, A ;

Book, LD ;

Xie, XS .

JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (07) :1277-1280

[9] Safety Assessment by Multiphoton Fluorescence/Second Harmonic Generation/Hyper-Rayleigh Scattering Tomography of ZnO Nanoparticles Used in Cosmetic Products [J].

Darvin, M. E. ;

Koenig, K. ;

Kellner-Hoefer, M. ;

Breunig, H. G. ;

Werncke, W. ;

Meinke, M. C. ;

Patzelt, A. ;

Sterry, W. ;

Lademann, J. .

SKIN PHARMACOLOGY AND PHYSIOLOGY, 2012, 25 (04) :219-226

[10] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

← 1 2 3 4 →