Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts

被引:70
作者
Pessler, F
Pendergrast, PS
Hernandez, N
机构
[1] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724
[2] HOWARD HUGHES MED INST,COLD SPRING HARBOR,NY 11724
关键词
D O I
10.1128/MCB.17.7.3786
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The human immunodeficiency virus (HIV-1) promoter directs the synthesis of two classes of RNA molecules, short transcripts and full-length transcripts. The synthesis of short transcripts depends on a bipartite DNA element, the inducer of short transcripts (IST), located in large part downstream of the HIV-1 start site of transcription. IST does not require any viral product for function and is thought to direct the assembly of transcription complexes that are incapable of efficient elongation. Nothing is known, however, about the biochemical mechanisms that mediate IST function. Here, we report the identification and purification of a factor that binds specifically to the IST. This factor, FBI-1, recognizes a large bipartite binding site that coincides with the bipartite IST element. It is constituted as least in part by an 86-kDa polypeptide that can be specifically cross-linked to IST. FBI-1 also binds to promoter and attenuation regions of a number of cellular and viral transcription units that are regulated by a transcription elongation block. This observation, together with the observation that the binding of FBI-1 to IST mutants correlates with the ability of these mutants to direct IST function, suggests that FBI-1 may be involved in the establishment of abortive transcription complexes.
引用
收藏
页码:3786 / 3798
页数:13
相关论文
共 75 条
[1]  
[Anonymous], 1988, Antibodies: A Laboratory Manual
[2]   ORIENTATION-DEPENDENT TRANSCRIPTIONAL ACTIVATOR UPSTREAM OF A HUMAN U2 SNRNA GENE [J].
ARES, M ;
MANGIN, M ;
WEINER, AM .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (07) :1560-1570
[3]   MAZ-DEPENDENT TERMINATION BETWEEN CLOSELY SPACED HUMAN-COMPLEMENT GENES [J].
ASHFIELD, R ;
PATEL, AJ ;
BOSSONE, SA ;
BROWN, H ;
CAMPBELL, RD ;
MARCU, KB ;
PROUDFOOT, NJ .
EMBO JOURNAL, 1994, 13 (23) :5656-5667
[4]   DIFFERENTIAL EXPRESSION OF C-MYB MESSENGER-RNA IN MURINE-B LYMPHOMAS BY A BLOCK TO TRANSCRIPTION ELONGATION [J].
BENDER, TP ;
THOMPSON, CB ;
KUEHL, WM .
SCIENCE, 1987, 237 (4821) :1473-1476
[5]   SEQUENCE REQUIREMENTS FOR PREMATURE TERMINATION OF TRANSCRIPTION IN THE HUMAN C-MYC GENE [J].
BENTLEY, DL ;
GROUDINE, M .
CELL, 1988, 53 (02) :245-256
[6]   A BLOCK TO ELONGATION IS LARGELY RESPONSIBLE FOR DECREASED TRANSCRIPTION OF C-MYC IN DIFFERENTIATED HL60 CELLS [J].
BENTLEY, DL ;
GROUDINE, M .
NATURE, 1986, 321 (6071) :702-706
[7]   MAZ, A ZINC FINGER PROTEIN, BINDS TO C-MYC AND C2 GENE-SEQUENCES REGULATING TRANSCRIPTIONAL INITIATION AND TERMINATION [J].
BOSSONE, SA ;
ASSELIN, C ;
PATEL, AJ ;
MARCU, KB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) :7452-7456
[8]   Activator-dependent regulation of transcriptional pausing on nucleosomal templates [J].
Brown, SA ;
Imbalzano, AN ;
Kingston, RE .
GENES & DEVELOPMENT, 1996, 10 (12) :1479-1490
[9]   THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TAT PROTEIN TRANSACTIVATES TUMOR-NECROSIS-FACTOR BETA-GENE EXPRESSION THROUGH A TAR-LIKE STRUCTURE [J].
BUONAGURO, L ;
BUONAGURO, FM ;
GIRALDO, G ;
ENSOLI, B .
JOURNAL OF VIROLOGY, 1994, 68 (04) :2677-2682
[10]   PROTEIN INTERACTIONS WITH DNA ELEMENTS IN VARIANT EQUINE INFECTIOUS-ANEMIA VIRUS ENHANCERS AND THEIR IMPACT ON TRANSCRIPTIONAL ACTIVITY [J].
CARVALHO, M ;
KIRKLAND, M ;
DERSE, D .
JOURNAL OF VIROLOGY, 1993, 67 (11) :6586-6595