TGF-β1 immobilized tri-co-polymer for articular cartilage tissue engineering

被引:41
作者
Chou, CH
Cheng, WTK
Lin, CC
Chang, CH
Tsai, CC
Lin, FH [1 ]
机构
[1] Natl Taiwan Univ, Inst Biomed Engn, Coll Med, Taipei 100, Taiwan
[2] Natl Taiwan Univ, Coll Engn, Taipei 100, Taiwan
[3] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Dept Biomed Engn, Taipei 100, Taiwan
[4] Natl Taiwan Univ, Dept Anim Sci, Taipei 10772, Taiwan
[5] Far Eastern Mem Hosp, Div Orthoped, Dept Surg, Taipei, Taiwan
[6] Far Eastern Mem Hosp, Dept Pathol, Taipei, Taiwan
关键词
TGF-beta; 1; gelatin; chondroitin-6-sulfate; hyaluronic acid; carbodiimide; articular cartilage; tissue engineering;
D O I
10.1002/jbm.b.30432
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Tri-co-polymer with composition of gelatin, hyaluronic acid and chondroitin-6-sulfate has been used to mimic the cartilage extracellular matrix as scaffold for cartilage tissue engineering. In this study, we try to immobilize TGF-beta 1 onto the surface of the tri-co-polymer sponge to suppress the undesired differentiation during the cartilage growth in vitro. The scaffold was synthesized with a pore size in a range of 300-500 mu m. TGF-beta 1 was immobilized on the surface of the tri-co-polymer scaffold with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimid (EDC) as a crosslinking agent. Tri-co-polymer scaffolds with and without TGF-beta 1 were seeded with porcine chondrocytes and cultured in a spinner flask for 2, 4, and 6 weeks. The chondrocytes were characterized by the methods of immunohistochemical staining with anti-type II collagen and anti-S-100 protein monoclonal antibody, and RT-PCR. After culturing for 4 weeks, chondrocytes showed positive in S-100 protein, Alcian blue, and type II collagen for the scaffold with TGF-beta 1 immobilization. There is no observed type I and type X collagen expression in the scaffolds from the observation of RT-PCR. In addition, the scaffold without TGF-beta 1 immobilization, type X collagen, can be detected after cultured for 2 weeks. Type I collagen was progressively expressed after 4 weeks. These results can conclude that TGF-beta 1 immobilized scaffold can suppress chondrocytes toward prehypertrophic chondrocytes and osteollineage cells. The tri-co-polymer sponge with TGF-beta 1 immobilization should have a great potential in cartilage tissue engineering in the future. (c) 2006 Wiley Periodicals, Inc.
引用
收藏
页码:338 / 348
页数:11
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