PCOS Is Associated with Increased CD11c Expression and Crown-Like Structures in Adipose Tissue and Increased Central Abdominal Fat Depots Independent of Obesity

被引:66
作者
Huang, Zhi Hua [1 ]
Manickam, Buvana [1 ]
Ryvkin, Victoria [1 ]
Zhou, Xiaohong Joe [2 ,3 ]
Fantuzzi, Giamila [4 ]
Mazzone, Theodore [5 ]
Sam, Susan [1 ]
机构
[1] Univ Illinois, Dept Med, Sect Endocrinol Diabet & Metab, Chicago, IL 60612 USA
[2] Univ Illinois, Dept Radiol, Chicago, IL 60612 USA
[3] Univ Illinois, Ctr Magnet Resonance Res, Chicago, IL 60612 USA
[4] Univ Illinois, Dept Kinesiol & Nutr, Chicago, IL 60612 USA
[5] NorthShore Univ Hlth Syst, Dept Med, Chicago, IL 60201 USA
基金
美国国家卫生研究院;
关键词
POLYCYSTIC-OVARY-SYNDROME; NECROSIS-FACTOR-ALPHA; INSULIN-RESISTANCE; MACROPHAGE INFILTRATION; ANTIINFLAMMATORY PHENOTYPE; GLUCOSE-TOLERANCE; BODY-COMPOSITION; APOE EXPRESSION; VISCERAL FAT; INFLAMMATION;
D O I
10.1210/jc.2012-2697
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Context: Adipose tissue macrophage (ATM) infiltration is a major pathway for obesity-induced insulin resistance but has not been studied as a mechanism for insulin resistance in PCOS. Objective: We tested whether polycystic ovary syndrome (PCOS) is associated with increased ATM infiltration, especially of inflammatory subtype identified by the CD11c marker. Design and Setting: We conducted a case-control study at an academic medical center in the United States. Participants and Interventions: Fourteen PCOS and 14 control women of similar age and body mass index (BMI) underwent a gluteal fat biopsy. Markers of ATM, integrins, TNF-alpha, and adiponectin, were analyzed by quantitative RT-PCR using a standard curve method. Crown-like structures (CLS) were identified by immunohistochemistry. Abdominal magnetic resonance imaging and frequently sampled iv glucose tolerance test were performed to assess abdominal fat and insulin sensitivity (SI). Main Outcome: Women with PCOS were compared with control women of similar age and BMI for ATM markers, CLS density, adipose tissue expression of inflammatory cytokines and adiponectin, SI, and abdominal fat depots. Results: Women with PCOS had an increase in CD11c expression (P = 0.03), CLS density (P = 0.001), alpha 5 expression (P = 0.009), borderline increase in TNF-alpha expression (P = 0.08), and a decrease in adiponectin expression (P = 0.02) in gluteal adipose tissue. Visceral (P = 0.009) and sc abdominal fat (P = 0.005) were increased in PCOS. SI was lower in PCOS (P = 0.008). Conclusions: PCOS is associated with an increase in CD11c expression and CLS density and a decrease in adiponectin expression in sc adipose tissue. Additionally, PCOS is associated with higher central abdominal fat depots independent of BMI. These alterations are present among mostly nonobese women and could represent mechanisms for insulin resistance. (J Clin Endocrinol Metab 98: E17-E24, 2013)
引用
收藏
页码:E17 / E24
页数:8
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