PREPARATIVE SEPARATION OF POLYPHENOLS FROM ARTICHOKE BY POLYAMIDE COLUMN CHROMATOGRAPHY AND HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

被引：7
作者：
Shu, Xikai [1 ]
Wang, Mei [1 ]
Liu, Daicheng [1 ]
Wang, Daijie [2 ]
Lin, Xiaojing [2 ]
Liu, Jianhua [2 ]
Wang, Xiao [2 ]
Huang, Luqi [2 ]
机构：
[1] Shandong Normal Univ, Coll Life Sci, Jinan 250014, Shandong, Peoples R China
[2] Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Shandong, Peoples R China
来源：
QUIMICA NOVA | 2013年 / 36卷 / 06期
关键词：
artichoke; HSCCC; polyphenols; PHENOLIC-COMPOUNDS; PURIFICATION; EXTRACT; ACID; L;
D O I：
10.1590/S0100-40422013000600017
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-beta-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-beta-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR).
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页码：836 / 839
页数：4
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