Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells

被引：2
作者：
Zhang, Hua
Li, Wei
Wu, YaoHong
Zhang, Shengquan
Li, Jie
Han, Letian
Chen, Haoyu
Wang, Ziyu
Shen, Cailiang
Zhang, Yinshun
Tao, Hui
机构：
[1] Department Of Orthopedics And Spine Surgery, The First Affiliated Hospital Of Anhui Medical University, Anhui, Hefei
[2] Department Of Orthopedics, Huaibei People's Hospital, Huaibei, Anhui
[3] Department Of Spinal Surgery, Ganzhou People's Hospital, Ganzhou, Jiangxi
[4] Department Of Biochemistry And Molecular Biology, School Of Basic Medical Sciences, Anhui Medical University, Anhui, Hefei
[5] Department Of Clinical Laboratory, The First Affiliated Hospital Of Anhui Medical University, Anhui, Hefei
来源：
STEM CELLS INTERNATIONAL | 2022年 / 2022卷
基金：
中国国家自然科学基金;
关键词：
INTERVERTEBRAL DISC; STROMAL CELLS; DEGENERATION; PROTEOGLYCANS; FIBROSUS; LOADS;
D O I：
10.1155/2022/1121064
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The expansion and maintenance of the NPMSC (nucleus pulposus mesenchymal stem cell) phenotype are considered as potential therapeutic tools for clinical applications in intervertebral disc tissue engineering and regenerative medicine. However, the harsh microenvironment within the intervertebral disc is the main limitation of its regeneration. The osmolarity of the intervertebral disc is higher than that of other tissues, which has an important influence on the biological characteristics of NPMSCs. In this study, we observed the effect of different osmolarities on the biological characteristics of human normal NPMSCs cultured in vitro and explored the role of osmolarity in intervertebral disc degeneration. Our data demonstrated that the change in osmotic pressure has an important effect on the biological activity of NPMSCs, and this effect may occur through the P16(INK4A)/Rb pathway. This study provides a theoretical basis for the future treatment of intervertebral disc degeneration.
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页数：15
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