Immunogenicity of monococcum wheat in celiac patients

被引:47
作者
Gianfrani, Carmen [1 ]
Maglio, Mariatonia [2 ,3 ]
Aufiero, Vera Rotondi [1 ]
Camarca, Alessandra [1 ]
Vocca, Immacolata [1 ]
Iaquinto, Gaetano [4 ]
Giardullo, Nicola [4 ]
Pogna, Norberto [5 ]
Troncone, Riccardo [2 ,3 ,6 ]
Auricchio, Salvatore [2 ,3 ]
Mazzarella, Giuseppe [1 ]
机构
[1] CNR, Inst Food Sci, I-83100 Avellino, Italy
[2] Univ Naples Federico II, Dept Paediat, Naples, Italy
[3] Univ Naples Federico II, European Lab Invest Food Induced Dis, Naples, Italy
[4] San G Moscati Hosp, Gastroenterol & Digest Endoscopy Serv, Avellino, Italy
[5] CRAQCE, Unit Cereal Qual, Rome, Italy
[6] King Saud Univ, Riyadh, Saudi Arabia
关键词
T-CELL EPITOPES; DISEASE PATIENTS; TRITICUM-MONOCOCCUM; GLUTEN PEPTIDES; GLIADIN; TOXICITY; RESPONSES; PROTEIN; MUCOSA;
D O I
10.3945/ajcn.112.040485
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Background: Research is intense to find wheat of low or null toxicity for patients with celiac disease (CD). Among candidates, there are diploid wheat species. Objective: We compared the immunological properties of 2 lines of diploid monococcum wheat (Tritium: monococcum ssp. monococcum). Monlis and ID331. with those of common wheat (Triticum aestivum). Design: Interferon-gamma production and the proliferation of intestinal gliadin-specific T cell lines and clones were measured as evidence of T cell activation by peptic and tryptic (PT) digests of gliadins from 2 monococcum lines. Furthermore, organ cultures of jejunal biopsies from 28 CD patients were set up to assess the effects of PT gliadin on innate and adaptive immune response by using immunohistochemistry. Results: Monlis and ID331 induced interferon-gamma production and proliferation in celiac mucosal T cells. In organ cultures, Monlis PT digest induced a significant increase of IL-15 epithelial expression and crypt enterocyte proliferation, whereas ID331 had no effect. Both monococcum lines caused intraepithelial T cell infiltration and lamina propria T cell activation. Conclusions: Our data show that the monococcum lines Monlis and ID331 activate the CD T cell response and suggest that these lines are toxic for celiac patients. However, ID331 is likely to be less effective in inducing CD because of its inability to activate the innate immune pathways. Am J Clin Nutr 2012;96:1339-45.
引用
收藏
页码:1339 / 1345
页数:7
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