A self-filling microfluidic device for noninvasive and time-resolved single red blood cell experiments

被引:9
作者
Gollner, Michael [1 ]
Toma, Adriana C. [1 ]
Strelnikova, Natalja [1 ]
Deshpande, Siddharth [1 ,2 ]
Pfohl, Thomas [1 ,3 ]
机构
[1] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland
[2] Delft Univ Technol, Dept Bionanosci, NL-2628 CJ Delft, Netherlands
[3] Univ Basel, Biomat Sci Ctr BMC, CH-4123 Allschwil, Switzerland
来源
BIOMICROFLUIDICS | 2016年 / 10卷 / 05期
基金
瑞士国家科学基金会;
关键词
RAMAN-SPECTROSCOPY; MEMBRANE; ERYTHROCYTES; HEMOGLOBIN; POLYDIMETHYLSILOXANE; FLUCTUATIONS; FABRICATION; EXCITATION; MECHANICS; TWEEZERS;
D O I
10.1063/1.4966212
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Existing approaches to red blood cell (RBC) experiments on the single-cell level usually rely on chemical or physical manipulations that often cause difficulties with preserving the RBC's integrity in a controlled microenvironment. Here, we introduce a straightforward, self-filling microfluidic device that autonomously separates and isolates single RBCs directly from unprocessed human blood samples and confines them in diffusion-controlled microchambers by solely exploiting their unique intrinsic properties. We were able to study the photo-induced oxygenation cycle of single functional RBCs by Raman microscopy without the limitations typically observed in optical tweezers based methods. Using bright-field microscopy, our non-invasive approach further enabled the time-resolved analysis of RBC flickering during the reversible shape evolution from the discocyte to the echinocyte morphology. Due to its specialized geometry, our device is particularly suited for studying the temporal behavior of single RBCs under precise control of their environment that will provide important insights into the RBC's biomedical and biophysical properties. Published by AIP Publishing.
引用
收藏
页数:11
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