Mitofusin-2 Independent Juxtaposition of Endoplasmic Reticulum and Mitochondria: An Ultrastructural Study

被引：186

作者：

Cosson, Pierre ^{[1
]}

Marchetti, Anna ^{[1
]}

Ravazzola, Mariella ^{[1
]}

Orci, Lelio ^{[1
]}

机构：

[1] Ctr Med Univ Geneva, Dept Cell Physiol & Metab, Geneva Fac Med, Geneva, Switzerland

来源：

PLOS ONE | 2012年 / 7卷 / 09期

基金：

瑞士国家科学基金会;

关键词：

ELECTRON TOMOGRAPHY; COLOCALIZATION; ORGANIZATION; MICROSCOPY; CHANNELS; FUSION; LINE; ER;

D O I：

10.1371/journal.pone.0046293

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Besides its role in controlling the morphology of mitochondria, mitofusin-2 has been proposed to tether mitochondria to the endoplasmic reticulum (ER), based largely on light microscopic analysis. In this study we have examined by electron microscopy the organization of ER and mitochondria in cells expressing or not mitofusin-2. Contrary to previous studies, we observed that loss of mitofusin-2 increased ER-mitochondria juxtaposition. These results suggest that mitofusin-2 does not play a critical role in the juxtapostion of ER and mitochondria, and highlight the essential role of ultrastructural analysis to visualize and measure contact between two intracellular compartments.

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