In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy

被引：19

作者：

Edward, Kert ^{[1
]}

Qiu, Suimin ^{[2
,5
]}

Resto, Vicente ^{[3
,5
]}

McCammon, Susan ^{[3
,5
]}

Vargas, Gracie ^{[1
,4
,5
]}

机构：

[1] Univ Texas Med Branch, Ctr Biomed Engn, Galveston, TX 77555 USA

[2] Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA

[3] Univ Texas Med Branch, Dept Otolaryngol, Galveston, TX 77555 USA

[4] Univ Texas Med Branch, Dept Neurosci & Cell biol, Galveston, TX 77555 USA

[5] Univ Texas Med Branch, Ctr Canc Head & Neck, Galveston, TX 77555 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2012年 / 3卷 / 07期

基金：

美国国家卫生研究院;

关键词：

HIGH-GRADE DYSPLASIA; MULTIPHOTON MICROSCOPY; FLUORESCENCE SPECTROSCOPY; EXCITATION WAVELENGTHS; BARRETTS-ESOPHAGUS; FIBEROPTIC PROBE; AUTOFLUORESCENCE SPECTROSCOPY; 2-PHOTON-EXCITED FLUORESCENCE; 2ND-HARMONIC GENERATION; COHERENCE TOMOGRAPHY;

D O I：

10.1364/BOE.3.001579

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optical spectroscopy has proven to be a powerful technique for studying neoplastic transformation in epithelial tissue. Since specific intra-layer precancerous changes originate in the stratified layers of the oral mucosa, layer-resolved analysis will likely improve both our understanding of the mechanism of premalignant transformation, and clinical diagnostic outcomes. However, the native fluorescence signal in linear spectroscopy typically originates from a multi-layered focal volume. In this study, nonlinear spectroscopy was exploited for in vivo layer-resolved discrimination between normal and dysplastic tissue for the first time. Our results revealed numerous intra-layer specific differences. (C) 2012 Optical Society of America

引用

页码：1579 / 1593

页数：15

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