Detection of Creatine in Rat Muscle by FTIR Spectroscopy

There is a current lack of clarity regarding the use of Fourier-transform infrared spectroscopy (FT-IR) to evaluate intramuscular concentrations of creatine (Cr). Thus, the aim of this study was to assess the FT-IR spectral features of tibialis anterior muscle in rats submitted in conditions that were expected to perturb the Cr pool. First, an experiment was performed to ensure that FT-IR was able to detect the Cr intramuscular in sedentary and supplemented rats (Experiment 1). The effect of physical exercise on spectral muscle features was then examined, especially in relation to the spectroscopy markers (Experiment 2). Using pure Cr (control), it was possible to verify that only the peaks centered at 1308 and 1396 cm(-1) of all the spectra showed the same peak positions, indicating these FT-IR shifts as indirect markers of Cr intramuscular content. Experiment 2 revealed a higher Cr content for the Cr-supplemented and exercised animals than the rats of other groups. In conclusion, it was demonstrated that FT-IR spectroscopy using 1396 cm(-1) and mainly 1308 band was able to monitor Cr muscle content in rats sedentary, Cr-supplemented, and submitted to physical training. Besides, FT-IR could be a feasible method for the nondestructive assessment of Cr skeletal muscle content.