Detection of multi-color fluorescent objects with single photon spectrometer

被引:8
作者
Gudkov, Dmytro [1 ]
Gavrilov, Dmitri [1 ]
Tsupryk, A. [1 ]
Tovkach, Ivan [1 ]
Gudkov, George [1 ]
Kosobokova, Olga [1 ]
Cherevishnik, Marina [1 ]
Gorbovitski, Boris [2 ]
Gorfinkel, Vera [1 ]
机构
[1] SUNY Stony Brook, Dept Elect & Comp Engn, Stony Brook, NY 11794 USA
[2] ABMM Corp, Stony Brook, NY USA
关键词
Spectrometer; Fluorescence detection; Single photon counting; Quantum dots; Beads; ENHANCED RAMAN-SCATTERING; LIBRARIES; COLLOIDS; SYSTEM;
D O I
10.1016/j.bios.2012.07.013
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Single photon counting is the most sensitive and accurate method for detection of very weak fluorescent signals obtained in many applications such as DNA sequencing, detection of biological reporters on micro-beads, detection of droplets in micro-fluidic systems, etc. In this paper we describe the use of single photon spectrometer for detection and characterization of very weak multicolor fluorescence produced by mixtures of various fluorescent dyes and quantum dots. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:152 / 155
页数:4
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