Hepatocytes transduced with human TERT gene acquire a prolonged lifespan in culture and retain permissiveness to hepatitis B virus

被引：0
作者：
Lv, L. P. [1 ]
Kong, Y. L. [2 ]
Yan, F. [1 ]
Ma, P. [1 ]
Zhou, X. P. [1 ]
Zhang, Y. Y. [1 ]
Wen, D. Q. [3 ]
Yu, X. L. [1 ]
Zhang, H. Y. [2 ]
Zhou, H. [1 ]
Xu, J. B. [1 ]
机构：
[1] Beijing Inst Transfus Med, Beijing 100850, Peoples R China
[2] Gen Hosp Air Force, Beijing 100142, Peoples R China
[3] Air Force Inst Aviat Med, Beijing 100036, Peoples R China
来源：
ACTA VIROLOGICA | 2013年 / 57卷 / 03期
基金：
北京市自然科学基金;
关键词：
hepatitis B virus; hepatocyte; TERT; transduction; primary; CELL LINE; INFECTION; REPLICATION; TELOMERASE; PROTEIN; LIVER;
D O I：
10.4149/av_2013_03_305
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Hepatitis B virus (HBV) can be propagated in vitro in primary cultures of human hepatocytes and some stable hepatoma cell lines maintained under specific conditions. The lack of simple and non-neoplastic cell culture systems for HBV has hampered the analysis of virus life cycle and development of antiviral compounds. In this study, we succeeded in prolonging the lifespan of human hepatocytes in primary culture by transducing them with human telomerase reverse transcriptase (hTERT) gene. The transgenic cells expressed hTERT constitutively and propagated HBV up to 5x10(5) DNA copies/ml for 28 days.
引用
收藏
页码：305 / 311
页数：7
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