A v-SNARE implicated in intra-Golgi transport

被引：95
作者：
Nagahama, M
Orci, L
Ravazzola, M
Amherdt, M
Lacomis, L
Tempst, P
Rothman, JE
Sollner, TH
机构：
[1] MEM SLOAN KETTERING CANC CTR,CELLULAR BIOCH & BIOPHYS PROGRAM,NEW YORK,NY 10021
[2] MEM SLOAN KETTERING CANC CTR,PROGRAM MOLEC BIOL,NEW YORK,NY 10021
[3] UNIV GENEVA,CTR MED,DEPT MORPHOL,INST HISTOL & EMBRYOL,CH-1211 GENEVA 4,SWITZERLAND
来源：
JOURNAL OF CELL BIOLOGY | 1996年 / 133卷 / 03期
关键词：
D O I：
10.1083/jcb.133.3.507
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, a Golgi SNARE of 28 kD, is efficiently packaged into Golgi-derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles.
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页码：507 / 516
页数：10
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