Rapid Identification of Microorganisms Isolated from Pickled Vegetables by MALDI-TOF Mass Spectrometry

被引：0

作者：

Tan Zhi-lei ^{[1
]}

Wei Yu-Qiao ^{[1
]}

Liang Shuang ^{[1
]}

Zhang Ran ^{[1
]}

Liu Miao ^{[1
]}

Jia Shi-Ru ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Sch Bioengn, Tianjin 300457, Peoples R China

来源：

ADVANCES IN MANUFACTURING SCIENCE AND ENGINEERING, PTS 1-4 | 2013年 / 712-715卷

关键词：

MALDI-TOF MS; 16S rRNA; Biotyper; Rapid microorganism identification;

D O I：

10.4028/www.scientific.net/AMR.712-715.494

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Matrix-assisted laser desorption/ionization time-off light mass spectrometry (MALDI-TOF MS) is increasingly used as a microbial diagnostic method for species identification of pathogens. However, MALDI-TOF MS identification of food bacteria was seldom reported. Ten strains isolated from different pickled vegetables were rapid identified by MALDI-TOF MS. The results of MALDI-TOF MS were confirmed by 16S rDNA sequencing method. Different score values in MALDI-TOF MS revealed nine Leuconostoc mesenteroides and one Staphylococcus. Identification success at the species and genus levels was 90% (9/10) and 100% (10/10), respectively. 16S rDNA sequencing results showed that nine stains were identified as Leuconostoc mesenteroides and one was Staphylococcus saprophyticus. Results obtained demonstrate that MALDI-TOFMS is a promising method for discriminating and identifying food bacteria.

引用

页码：494 / 497

页数：4

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