Localization of peripheral autonomic neurons innervating the boar urinary bladder trigone and neurochemical features of the sympathetic component

被引:26
作者
Ragionieri, L. [1 ]
Botti, M. [1 ]
Gazza, F. [1 ]
Sorteni, C. [2 ]
Chiocchetti, R. [2 ]
Clavenzani, P. [2 ]
Minelli, L. Bo [1 ]
Panu, R. [1 ]
机构
[1] Univ Parma, Dept Vet Sci, I-43100 Parma, Italy
[2] Univ Bologna, Dept Vet Med Sci, I-40126 Bologna, Italy
来源
EUROPEAN JOURNAL OF HISTOCHEMISTRY | 2013年 / 57卷 / 02期
关键词
immunohistochemistry; retrograde tracing; peripheral autonomic neurons; urinary bladder trigone; pig; SUPERIOR CERVICAL-GANGLION; DOUBLE-LABELING IMMUNOHISTOCHEMISTRY; VASOACTIVE INTESTINAL POLYPEPTIDE; INFERIOR MESENTERIC GANGLION; NITRIC-OXIDE SYNTHASE; RETRACTOR CLITORIDIS MUSCLE; BETA-ADRENOCEPTOR SUBTYPES; ENTERIC NERVOUS-SYSTEM; DORSAL-ROOT GANGLIA; TRACT SMOOTH-MUSCLE;
D O I
10.4081/ejh.2013.e16
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The urinary bladder trigone (UBT) is a limited area through which the majority of vessels and nerve fibers penetrate into the urinary bladder and where nerve fibers and intramural neurons are more concentrated. We localized the extramural post-ganglionic autonomic neurons supplying the porcine UBT by means of retrograde tracing (Fast Blue, FB). Moreover, we investigated the phenotype of sympathetic trunk ganglia (STG) and caudal mesenteric ganglia (CMG) neurons positive to FB (FB+) by coupling retrograde tracing and double-labeling immunofluorescence methods. A mean number of 1845.1 +/- 259.3 FB+ neurons were localized bilaterally in the L1-S3 STG, which appeared as small pericarya (465.6 +/- 82.7 mu m(2)) mainly localized along an edge of the ganglion. A large number (4287.5 +/- 1450.6) of small (476.1 +/- 103.9 mu m(2)) FB+ neurons were localized mainly along a border of both CMG. The largest number (4793.3 +/- 1990.8) of FB+ neurons was observed in the pelvic plexus (PP), where labeled neurons were often clustered within different microganglia and had smaller soma cross-sectional area (374.9 +/- 85.4 mu m(2)). STG and CMG FB+ neurons were immunoreactive (IR) for tyrosine hydroxylase (TH) (66 +/- 10.1% and 52.7 +/- 8.2%, respectively), dopamine beta-hydroxylase (D beta H) (62 +/- 6.2% and 52 +/- 6.2%, respectively), neuropeptide Y (NPY) (59 +/- 8.2% and 65.8 +/- 7.3%, respectively), calcitonin-gene-related peptide (CGRP) (24.1 +/- 3.3% and 22.1 +/- 3.3%, respectively), substance P (SP) (21.6 +/- 2.4% and 37.7 +/- 7.5%, respectively), vasoactive intestinal polypeptide (VIP) (18.9 +/- 2.3% and 35.4 +/- 4.4%, respectively), neuronal nitric oxide synthase (nNOS) (15.3 +/- 2% and 32.9 +/- 7.7%, respectively), vesicular acetylcholine transporter (VAChT) (15 +/- 2% and 34.7 +/- 4.5%, respectively), leuenkephalin (LENK) (14.3 +/- 7.1% and 25.9 +/- 8.9%, respectively), and somatostatin (SOM) (12.4 +/- 3% and 31.8 +/- 7.3%, respectively). UBT-projecting neurons were also surrounded by VAChT-, CGRP-, LENK-, and nNOS-IR fibers. The possible role of these neurons and fibers in the neural pathways of the UBT is discussed.
引用
收藏
页码:93 / 105
页数:13
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