Label-Free Quantitative Imaging of Cholesterol in Intact Tissues by Hyperspectral Stimulated Raman Scattering Microscopy

被引：90
作者：
Wang, Ping [1 ]
Li, Junjie [2 ]
Wang, Pu [1 ]
Hu, Chun-Rui [1 ]
Zhang, Delong [3 ]
Sturek, Michael [4 ]
Cheng, Ji-Xin [1 ]
机构：
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47906 USA
[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47906 USA
[3] Purdue Univ, Dept Chem, W Lafayette, IN 47906 USA
[4] Indiana Univ, Sch Med, Dept Cellular & Integrat Physiol, Indianapolis, IN 46202 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 49期
基金：
美国国家卫生研究院;
关键词：
cholesterol; lipids; stimulated Raman scattering; tissue analysis; vibrational spectroscopy; MULTIPLEX CARS MICROSCOPY; MULTIVARIATE CURVE RESOLUTION; LIPID DROPLETS; ATHEROSCLEROTIC PLAQUE; IN-VIVO; CRYSTALS; STATE;
D O I：
10.1002/anie.201306234
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：13042 / 13046
页数：5
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