Label-Free Quantitative Imaging of Cholesterol in Intact Tissues by Hyperspectral Stimulated Raman Scattering Microscopy

被引：90

作者：

Wang, Ping ^{[1
]}

Li, Junjie ^{[2
]}

Wang, Pu ^{[1
]}

Hu, Chun-Rui ^{[1
]}

Zhang, Delong ^{[3
]}

Sturek, Michael ^{[4
]}

Cheng, Ji-Xin ^{[1
]}

机构：

[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47906 USA

[2] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47906 USA

[3] Purdue Univ, Dept Chem, W Lafayette, IN 47906 USA

[4] Indiana Univ, Sch Med, Dept Cellular & Integrat Physiol, Indianapolis, IN 46202 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 49期

基金：

美国国家卫生研究院;

关键词：

cholesterol; lipids; stimulated Raman scattering; tissue analysis; vibrational spectroscopy; MULTIPLEX CARS MICROSCOPY; MULTIVARIATE CURVE RESOLUTION; LIPID DROPLETS; ATHEROSCLEROTIC PLAQUE; IN-VIVO; CRYSTALS; STATE;

D O I：

10.1002/anie.201306234

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：13042 / 13046

页数：5

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