Usefulness of capillary electrophoresis-based multiplex PCR assay for species-specific identification of Candida spp.

被引：6
|
作者：
Mallus, F. [1 ]
Martis, S. [1 ]
Serra, C. [2 ]
Loi, G. [2 ]
Camboni, T. [1 ]
Manzin, A. [1 ]
机构：
[1] Univ Cagliari, Dept Biomed Sci, Microbiol & Virol Unit, I-09042 Monserrato, CA, Italy
[2] Univ Cagliari, Dept Med Sci, I-09042 Monserrato, CA, Italy
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2013年 / 92卷 / 02期
关键词：
Candida spp; Multiplex PCR; Species-specific identification; Capillary electrophoresis; INVASIVE CANDIDIASIS; RAPID IDENTIFICATION; DIAGNOSIS; CULTURES; SPP;
D O I：
10.1016/j.mimet.2012.11.022
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The study evaluated the performances of a commercial multiplex PCR assay, the Seegene Seeplex STI Master Panel 3, for Candida spp. identification. Eighty clinical strains of Candida spp. were identified with this system and a homemade multiplex PCR assay. The results were also compared with those obtained with two phenotypic methods. The study provided a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the detection of amplified products. The Seeplex assay was found to be a rapid and useful method for identifying large numbers of yeast isolates in the clinical laboratory context. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：150 / 152
页数：3
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