Usefulness of capillary electrophoresis-based multiplex PCR assay for species-specific identification of Candida spp.

被引：6

作者：

Mallus, F. ^{[1
]}

Martis, S. ^{[1
]}

Serra, C. ^{[2
]}

Loi, G. ^{[2
]}

Camboni, T. ^{[1
]}

Manzin, A. ^{[1
]}

机构：

[1] Univ Cagliari, Dept Biomed Sci, Microbiol & Virol Unit, I-09042 Monserrato, CA, Italy

[2] Univ Cagliari, Dept Med Sci, I-09042 Monserrato, CA, Italy

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2013年 / 92卷 / 02期

关键词：

Candida spp; Multiplex PCR; Species-specific identification; Capillary electrophoresis; INVASIVE CANDIDIASIS; RAPID IDENTIFICATION; DIAGNOSIS; CULTURES; SPP;

D O I：

10.1016/j.mimet.2012.11.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The study evaluated the performances of a commercial multiplex PCR assay, the Seegene Seeplex STI Master Panel 3, for Candida spp. identification. Eighty clinical strains of Candida spp. were identified with this system and a homemade multiplex PCR assay. The results were also compared with those obtained with two phenotypic methods. The study provided a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the detection of amplified products. The Seeplex assay was found to be a rapid and useful method for identifying large numbers of yeast isolates in the clinical laboratory context. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：150 / 152

页数：3

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