Neutron scattering: a tool to detect in vivo thermal stress effects at the molecular dynamics level in micro-organisms

被引：23

作者：

Marty, Vincent ^{[1
,2
]}

Jasnin, Marion ^{[3
]}

Fabiani, Elisa ^{[2
]}

Vauclare, Pierre ^{[1
]}

Gabel, Frank ^{[4
]}

Trapp, Marcus ^{[2
,3
]}

Peters, Judith ^{[2
,3
]}

Zaccai, Giuseppe ^{[1
,3
]}

Franzetti, Bruno ^{[1
]}

机构：

[1] CNRS, Inst Biol Struct, F-38027 Grenoble, France

[2] Univ Grenoble 1, Inst Biol Struct, F-38000 Grenoble, France

[3] Inst Max Von Laue Paul Langevin, F-38042 Grenoble 9, France

[4] CEA, Inst Biol Struct, F-38054 Grenoble, France

来源：

JOURNAL OF THE ROYAL SOCIETY INTERFACE | 2013年 / 10卷 / 82期

关键词：

protein folding; stress response; cell biology; MACROMOLECULAR DYNAMICS; PROTEIN; ADAPTATION; TRANSITION; MOTIONS;

D O I：

10.1098/rsif.2013.0003

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In vivo molecular dynamics in Halobacterium salinarum cells under stress conditions was measured by neutron scattering experiments coupled with microbiological characterization. Molecular dynamics alterations were detected with respect to unstressed cells, reflecting a softening of protein structures consistent with denaturation. The experiments indicated that the neutron scattering method provides a promising tool to study molecular dynamics modifications in the proteome of living cells induced by factors altering protein folds.

引用

页数：6

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