Chiral analysis of metoprolol and two of its metabolites, α-hydroxymetoprolol and deaminated metoprolol, in wastewater using liquid chromatography-tandem mass spectrometry

被引:37
作者
Barclay, Victoria K. H. [1 ]
Tyrefors, Niklas L. [1 ]
Johansson, I. Monika [1 ,2 ]
Pettersson, Curt E. [1 ]
机构
[1] Uppsala Univ, Div Analyt Pharmaceut Chem, SE-75123 Uppsala, Sweden
[2] Med Prod Agcy, SE-75103 Uppsala, Sweden
关键词
Metoprolol; Metabolites; Wastewater; Enantiomeric separation; Chiral stationary phase; LC-MS; SEWAGE-TREATMENT PLANTS; SOLID-PHASE EXTRACTION; STATIONARY-PHASE; ENANTIOSELECTIVE DETERMINATION; PHARMACEUTICAL RESIDUES; TRACE ANALYSIS; BETA-BLOCKERS; RAT-PLASMA; SEPARATION; PHARMACOKINETICS;
D O I
10.1016/j.chroma.2012.09.090
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A LC-MS/MS method for the chiral separation of metoprolol and two of its main metabolites, alpha-hydroxymetoprolol (alpha-OH-Met) and deaminated metoprolol (COOH-Met), in environmental water samples has been developed. The target bases, metoprolol and alpha-OH-Met, as well as the acidic metabolite (COOH-Met) were extracted from water samples by a solid phase extraction method employing Oasis HLB cartridges. The extraction recoveries were >= 73% for all compounds in surface water. Four different types of chiral stationary phases were investigated for the separation of the eight stereoisomers of metaprolol and its metabolites, Chiralcel OD-H, Chirobiotic V. Chiral AGP and Chiral CBH. In the final method, the enantiomers of metoprolol and four stereoisomers of alpha-OH-Met were separated using Chiral CBH, the enantiomers of COOH-Met were separated employing Chiral AGP. The analytes were detected in SRM mode by triple quadrupole mass spectrometry. The method was applied for the chiral analysis of the analytes in treated wastewater samples from Uppsala, Sweden. The enantiomers and diastereoisomers of alpha-OH-Met were detected and analyzed in the samples. The concentrations of the three first eluting stereoisomers of alpha-OH-Met were between 54 and 61 pM. Interestingly, the last eluting stereoisomer was found to be present at a concentration of 151 pM at the same sampling occasion. This is, to the best of the authors' knowledge, the first time the stereoisomers of alpha-OH-Met have been detected in wastewater samples. The enantiomers of metoprolol were determined to be 1.77 and 1.86 nM in the same matrix. The enantiomers of COOH-Met were not detected above the method detection limit (42 pM) in treated wastewater samples. The developed LC-MS/MS methods were validated in wastewater samples. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:208 / 217
页数:10
相关论文
共 54 条
[1]   Evaluation of generic chiral liquid chromatography screens for pharmaceutical analysis [J].
Anderson, ME ;
Aslan, D ;
Clarke, A ;
Roeraade, J ;
Hagman, G .
JOURNAL OF CHROMATOGRAPHY A, 2003, 1005 (1-2) :83-101
[2]   RETENTION PROCESSES ON ALPHA-1-ACID GLYCOPROTEIN-BONDED STATIONARY PHASE [J].
ARVIDSSON, E ;
JANSSON, SO ;
SCHILL, G .
JOURNAL OF CHROMATOGRAPHY, 1992, 591 (1-2) :55-63
[3]   Trace analysis of fluoxetine and its metabolite norfluoxetine. Part II: Enantioselective quantification and studies of matrix effects in raw and treated wastewater by solid phase extraction and liquid chromatography-tandem mass spectrometry [J].
Barclay, Victoria K. H. ;
Tyrefors, Niklas L. ;
Johansson, I. Monika ;
Pettersson, Curt E. .
JOURNAL OF CHROMATOGRAPHY A, 2012, 1227 :105-114
[4]   Trace analysis of fluoxetine and its metabolite norfluoxetine. Part I: Development of a chiral liquid chromatography-tandem mass spectrometry method for wastewater samples [J].
Barclay, Victoria K. H. ;
Tyrefors, Niklas L. ;
Johansson, I. Monika ;
Pettersson, Curt E. .
JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (33) :5587-5596
[5]   Simultaneous analysis of several antiretroviral nucleosides in rat-plasma by high-performance liquid chromatography with UV using acetic acid/hydroxylamine buffer - Test of this new volatile medium-pH for HPLC-ESI-MS/MS [J].
Bezy, V ;
Morin, P ;
Couerbe, P ;
Leleu, G ;
Agrofoglio, L .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 821 (02) :132-143
[6]   Stereoselective analysis of metoprolol and its metabolites in rat plasma with application to oxidative metabolism [J].
Boralli, VB ;
Coelho, EB ;
Cerqueira, PM ;
Lanchote, VL .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 823 (02) :195-202
[7]  
BORG KO, 1975, ACTA PHARMACOL TOX, V36, P125
[9]   Direct liquid chromatographic enantioseparation of sotalol and other beta-blockers using an alpha(1)-acid glycoprotein-based chiral stationary phase [J].
Ceccato, A ;
Hubert, P ;
Crommen, J .
JOURNAL OF CHROMATOGRAPHY A, 1997, 760 (02) :193-203
[10]  
Cerqueira PM, 1999, CHIRALITY, V11, P591, DOI 10.1002/(SICI)1520-636X(1999)11:7<591::AID-CHIR12>3.0.CO