Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1

被引：143

作者：

Satouh, Yuhkoh ^{[2
]}

Inoue, Naokazu ^{[1
]}

Ikawa, Masahito ^{[1
]}

Okabe, Masaru ^{[1
]}

机构：

[1] Osaka Univ, Microbial Dis Res Inst, Suita, Osaka 5650871, Japan

[2] Osaka Univ, World Premier Int Immunol Frontier Res Ctr, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF CELL SCIENCE | 2012年 / 125卷 / 21期

关键词：

Acrosome reaction; Membrane fusion; Protein trafficking; Live-cell imaging; IZUMO1; Sperm-egg fusion; ACROSOMAL EXOCYTOSIS; PLASMA-MEMBRANE; PROTEIN IZUMO; GAMETE FUSION; FERTILIZATION; SPERMATOZOA; FERTILITY; MATRIX; CELL;

D O I：

10.1242/jcs.100867

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Gene disruption experiments have proven that the acrosomal protein IZUMO1 is essential for sperm-egg fusion in the mouse. However, despite its predicted function, it is not expressed on the surface of ejaculated spermatozoa. Here, we report the dynamics of diffusion of IZUMO1 from the acrosomal membrane to the sperm surface at the time of the acrosome reaction, visualized using a fluorescent protein tag. IZUMO1 showed a tendency to localize in the equatorial segment of the sperm surface after the acrosome reaction. This region is considered to initiate fusion with the oolemma. The moment of sperm-egg fusion and the dynamic movements of proteins during fusion were also imaged live. Translocation of IZUMO1 during the fertilization process was clarified, and a fundamental mechanism in mammalian fertilization is postulated.

引用

页码：4985 / 4990

页数：6

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