Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer

被引：4

作者：

Li, Xiaogang ^{[1
]}

Lu, Ping ^{[2
]}

Li, Bo ^{[1
]}

Zhang, Wangfu ^{[1
]}

Luo, Kaiyuan ^{[1
]}

机构：

[1] Kunming Med Univ, Dept Gen Surg, Affiliated Hosp 4, Kunming 650021, Yunnan, Peoples R China

[2] Kunming Med Univ, Dept Vasc Surg, Affiliated Hosp 4, Kunming 650021, Yunnan, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2013年 / 6卷 / 05期

关键词：

siodine-125; seeds; colorectal cancer; SFRP2; P16; methylation; DNA METHYLTRANSFERASES; IRRADIATION; EXPRESSION; SURGERY;

D O I：

10.3892/etm.2013.1298

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The current study investigated the effects of iodine-125 seeds on the gene methylation of SFRP2 and P16 in colorectal cancer. Mouse models of human colorectal cancer were randomly divided into an experimental group (n=25) and a control group (n=25). The control group was implanted with blank seeds (0 MBq) and the experimental group with iodine-125 seeds (14.8 MBq). At 20 days after implantation, the animals were sacrificed. The methylation levels of SFRP and P16 were detected using methylation-specific polymerase chain reactions (MSPs). Following iodine-125 seed irradiation, the level of SFRP, methylation decreased. The methylation index of the experimental group (0.67 +/- 0.05) was significantly lower than that of the control group (0.84 +/- 0.07; P<0.05). In the experimental group, 10 samples (40%) displayed methylation in the P16 promoter region compared with 14 (56%) in the control group, which was a significant difference (P<0.05). Iodine-125 seeds induce the downregulation of methylated tumor suppressor gene promoters, thereby inhibiting the proliferation and growth of tumor cells.

引用

页码：1225 / 1228

页数：4

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