DosR Regulates the Transcription of the Arginine Biosynthesis Gene Cluster by Binding to the Regulatory Sequences in Mycobacterium bovis Bacille Calmette-Guerin

被引：4

作者：

Cui, Yingying ^{[1
]}

Dang, Guanghui ^{[1
]}

Wang, Hui ^{[1
]}

Tang, Yiyi ^{[1
]}

Lv, Mingyue ^{[1
]}

Zang, Xinxin ^{[1
]}

Cai, Zhuming ^{[1
]}

Cui, Ziyin ^{[1
]}

Cao, Jun ^{[1
]}

Liu, Siguo ^{[1
,3
]}

Song, Ningning ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, Div Bacterial Dis, State Key Lab Vet Biotechnol, Harbin, Peoples R China

[2] Weifang Med Univ, Sch Life Sci & Technol, Bioengn Dept, Weifang, Peoples R China

[3] Chinese Acad Agr Sci, Harbin Vet Res Inst, Div Bacterial Dis, State Key Lab Vet Biotechnol, 678 Haping St, Harbin 150069, Peoples R China

来源：

DNA AND CELL BIOLOGY | 2022年 / 41卷 / 12期

基金：

中国国家自然科学基金; 黑龙江省自然科学基金;

关键词：

Mycobacterium bovis BCG; ChIP; DosR; transcriptional regulation; arginine synthesis; RESPONSE REGULATOR; DNA OPERATOR; TUBERCULOSIS; ACTIVATION; MECHANISM; DEVR; REPRESSOR; REGULON; COMPLEX; HELIX;

D O I：

10.1089/dna.2022.0282

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

l-Arginine serves as a carbon and nitrogen source and is critical for Mycobacterium tuberculosis (Mtb) survival in the host. Generally, ArgR acts as a repressor regulating arginine biosynthesis by binding to the promoter of the argCJBDFGH gene cluster. In this study, we report that the dormancy regulator DosR is a novel arginine regulator binding to the promoter region of argC (rv1652), which regulates arginine synthesis. Phosphorylation modification promoted DosR binding to a region upstream of the promoter. Cofactors, including arginine and metal ions, had an inhibitory effect on this association. Furthermore, DosR regulatory function relies on the interaction of the 167, 181, 182, and 197 amino acid residues with an inverse complementary sequence. Arginine also binds to DosR and directly affects its DNA-binding ability. Together, the results demonstrate that DosR acts as a novel transcriptional regulator of arginine synthesis in Mycobacterium bovis bacille Calmette-Guerin.

引用

页码：1063 / 1074

页数：12

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