Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat

被引：13

作者：

Lei, Jia ^{[1
]}

Zhou, Jiawen ^{[1
]}

Sun, Haojie ^{[1
]}

Wan, Wentao ^{[1
]}

Xiao, Jin ^{[1
]}

Yuan, Chunxia ^{[1
]}

Karafiatova, Miroslava ^{[2
]}

Dolezel, Jaroslav ^{[2
]}

Wang, Haiyan ^{[1
]}

Wang, Xiue ^{[1
]}

机构：

[1] Nanjing Agr Univ, Cytogenet Inst, State Key Lab Crop Genet & Germplasm Enhancement, JCIC MCP, Nanjing 210095, Jiangsu, Peoples R China

[2] Czech Acad Sci, Ctr Reg Hana Biotechnol & Agr Res, Inst Expt Bot, Slechtitelu 31, Olomouc 77900, Czech Republic

来源：

CROP JOURNAL | 2020年 / 8卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Chromosome identification; Haynaldia villosa; Oligonucleotide probes; Tandem DNA repeats; DASYPYRUM-VILLOSUM; RESISTANCE GENE; CHROMOSOMES; CHROMATIN; QUALITY; L;

D O I：

10.1016/j.cj.2020.02.008

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement. Owing to the limited number of probes available for fluorescence in situ hybridization (FISH), the resolution at which the karyotype of H. villosa can be characterized is poor, hampering accurate characterization of small segmental alien introgressions. We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H. villosa chromosome 6V (6VS). FISH with seven of them resulted in clear signals on H. villosa chromosomes. Using these, we constructed FISH karyotypes for H. villosa using oligo-6VS-1 and oligo-6VS-35 oligonucleotides and characterized the distribution of the two probes in five different H. villosa accessions. The new FISH probes can efficiently characterize H. villosa introgressions into wheat. (C) 2020 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

引用

页码：676 / 681

页数：6

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