The stromal cell-derived factor-1α dependent migration of human cord blood CD34+ haematopoietic stem and progenitor cells switches from protein kinase C (PKC)-α dependence to PKC-α independence upon prolonged culture in the presence of Flt3-ligand and interleukin-6

被引：7
作者：
Kasenda, Benjamin [1 ]
Kassmer, Susannah H. [1 ]
Niggemann, Bernd [1 ]
Schiermeier, Sven [2 ]
Hatzmann, Wolfgang [2 ]
Zaenker, Kurt S. [1 ]
Dittmar, Thomas [1 ]
机构：
[1] Univ Witten Herdecke, Inst Immunol, D-58448 Witten, Germany
[2] Univ Witten Herdecke, Dept Gynaecol, Marien Hosp, D-58448 Witten, Germany
来源：
BRITISH JOURNAL OF HAEMATOLOGY | 2008年 / 142卷 / 05期
关键词：
haematopoietic stem cells; cell movement; stromal cell-derived factor-1 alpha; protein kinase C-a alpha; cultivation;
D O I：
10.1111/j.1365-2141.2008.07256.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Addition of the inflammatory cytokine interleukin (IL)-6 to the culture medium of human cord blood haematopoietic stem and progenitor cells (HSPCs) has been shown to lead to an altered stromal cell-derived factor-1 alpha-dependent migratory phenotype. This study investigated whether this effect was attributed to a differential engagement of protein kinase C (PKC) isotypes. The migratory activity of both Flt3-ligand and Flt3-ligand/IL-6 cultured cord blood HSPCs was PKC-alpha dependent on day 1, but PKC-alpha independent after 5 d of cultivation. PKC-alpha expression was not down-regulated in cells cultured for 5 d indicating a switch of signalling molecules directing cell migration.
引用
收藏
页码：831 / 835
页数：5
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