The stromal cell-derived factor-1α dependent migration of human cord blood CD34+ haematopoietic stem and progenitor cells switches from protein kinase C (PKC)-α dependence to PKC-α independence upon prolonged culture in the presence of Flt3-ligand and interleukin-6

被引：7

作者：

Kasenda, Benjamin ^{[1
]}

Kassmer, Susannah H. ^{[1
]}

Niggemann, Bernd ^{[1
]}

Schiermeier, Sven ^{[2
]}

Hatzmann, Wolfgang ^{[2
]}

Zaenker, Kurt S. ^{[1
]}

Dittmar, Thomas ^{[1
]}

机构：

[1] Univ Witten Herdecke, Inst Immunol, D-58448 Witten, Germany

[2] Univ Witten Herdecke, Dept Gynaecol, Marien Hosp, D-58448 Witten, Germany

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2008年 / 142卷 / 05期

关键词：

haematopoietic stem cells; cell movement; stromal cell-derived factor-1 alpha; protein kinase C-a alpha; cultivation;

D O I：

10.1111/j.1365-2141.2008.07256.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Addition of the inflammatory cytokine interleukin (IL)-6 to the culture medium of human cord blood haematopoietic stem and progenitor cells (HSPCs) has been shown to lead to an altered stromal cell-derived factor-1 alpha-dependent migratory phenotype. This study investigated whether this effect was attributed to a differential engagement of protein kinase C (PKC) isotypes. The migratory activity of both Flt3-ligand and Flt3-ligand/IL-6 cultured cord blood HSPCs was PKC-alpha dependent on day 1, but PKC-alpha independent after 5 d of cultivation. PKC-alpha expression was not down-regulated in cells cultured for 5 d indicating a switch of signalling molecules directing cell migration.

引用

页码：831 / 835

页数：5

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